FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n02 gfp_15hpf_30apr.34200000033576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n02 gfp_15hpf_30apr.34200000033576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17890048
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG32300.012.67784443
TCTCGGT32900.012.03630841
CTCGGTG35350.011.58442
AGATCTC36600.011.55721938
GTAGATC37000.011.06741436
ATCTCGG40450.09.84535240
CTGCCGA41600.09.7895324
GACGAGT42400.09.76401629
CCGACGA41850.09.62352427
CGGTGGT43750.09.51415544
GGTGGTC42100.09.51293345
CGAGTGT46050.09.03896431
CTGACGC47200.08.91419818
ACGAGTG48400.08.8790130
TAGACCG32950.08.8092225
CGCTGCC47400.08.78152622
GATCTCG46900.08.77919839
GTATAAG107700.08.7546831
GACGCTG49800.08.71978420
TGACGCT50450.08.69673219
Produced by FastQC (version 0.11.2)