FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n02 gdna_4jun.3420000003355d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n02 gdna_4jun.3420000003355d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83358331
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT301300.022.79850845
CTGACGC435350.017.77347645
TCTGACG619750.012.52147344
CCTTTGG273100.011.8732321
CTTTGGG288250.010.569482
ATCTGAC757700.010.26551643
TAGACCG158800.010.102575
TATACCG171950.08.8458045
ATACACA2622900.08.83642145
GGGAAAC331350.08.7731716
TTTGGGA337500.08.69376853
AGACCGT181900.08.6833136
CATCTGA904200.08.639605542
TATACAC3287800.08.52758945
TACACAT1943850.08.332746545
GTATAGG265050.08.0313771
CGGGACT147050.07.89520510
TTGGGAA389000.07.849364
GTGTAGG206250.07.7462421
TACACTG312350.07.7078825
Produced by FastQC (version 0.11.2)