FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n02 dnfgfr_18hpf_4jun.34200000033540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n02 dnfgfr_18hpf_4jun.34200000033540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9779519
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT17550.015.89733741
TCGGTGG18450.015.24380943
CTCGGTG18700.014.55873442
CGGTGGT20700.013.80426444
CTGCCGA23850.011.88672124
GTAGATC23300.011.78104336
CCGACGA23300.011.78104327
ATCTCGG23200.011.63785940
AGATCTC25300.010.93866838
GCTGCCG26050.010.88285123
GCCGACG25850.010.88001126
CTGACGC25850.010.79297118
GTATAAG61550.010.602211
GACGAGT25650.010.43853229
ACGCTGC28300.010.41513121
CGCTGCC26650.010.38455222
CGAGTGT26450.010.20787731
GACGCTG29050.09.99133320
ACGAGTG27150.09.9446930
TGACGCT27850.09.93710219
Produced by FastQC (version 0.11.2)