FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n02 dnfgfr_15hpf_30apr.34200000033519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n02 dnfgfr_15hpf_30apr.34200000033519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24923413
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG35200.017.32222743
CTCGGTG38100.015.236032542
TCTCGGT38600.015.15525341
CGGTGGT47300.013.03366644
ATCTCGG52500.011.35700240
CTGCCGA52900.011.3136624
CCGACGA54650.010.91020327
GCTGCCG55500.010.8647323
GACGAGT53550.010.84020129
ACTACCC36350.010.6463818
GCCGACG57300.010.5234326
CGCTGCC57800.010.35456422
CTGACGC59800.010.34700718
AACCGCG36600.010.3277637
ACGCTGC60500.010.30154621
GACGCTG60900.010.23394520
TGACGCT60700.010.19359219
AGATCTC60550.010.18154138
GTAGATC59750.010.05426936
GCGGTAG30050.09.88340511
Produced by FastQC (version 0.11.2)