FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n01 lacz_18hpf_21may.341000000334f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n01 lacz_18hpf_21may.341000000334f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGA22.5316455696202533No Hit
GGTACGGTCAGGCATCCACGGCGCTTTAAAATAGTTGTTATAGATATTCAA11.2658227848101267No Hit
GTTGTATAAGATATCTATGAGTAAGTCTGGTGGTTCAAACCTTTTATGAAG11.2658227848101267No Hit
CTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATTAATGC11.2658227848101267No Hit
CGGGAAAGCAATCGACGGTAACTCTGATGACTGATGCTTTCCGCCGTCACC11.2658227848101267No Hit
CGATGGATTACGGGATTATAAAATAATAATTGAGAATTAGGAGAAAATTCC11.2658227848101267No Hit
ATCTGGGAAGATGTTGGTTGCAAGGTTCAAGGTCTTAAATATCCTGGTGAT11.2658227848101267No Hit
CGCTGCTGTAGACCGGCAGCCGTTTGCCGTTTTGGGTTACGAGATAATCCT11.2658227848101267No Hit
TGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGCAAAAA11.2658227848101267No Hit
GCGAAACGGTTCCTTTCTCCCGCAAGGGCTTGGCTATGGACCTGTCTCTTA11.2658227848101267No Hit
AAATGATTGGCGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGA11.2658227848101267No Hit
CAATACGTCAGGCCGACGTTTTCGCTCAGTGTAAAACGACCTAGGATGGTC11.2658227848101267No Hit
GTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCA11.2658227848101267No Hit
ACTGGTGAACCAAGATCGTGCCACTGCACTCCAGCCTGGCGACAGAGCGAG11.2658227848101267No Hit
TTATTACGTCATGCGAATTACGTCACAAATAAGAAACAATTTAAAGAAAGA11.2658227848101267No Hit
CTGCATAACTACATACCAGATTGAAAGTACCCGATCTCGTCCGATCTCGGA11.2658227848101267No Hit
GCAATGGAGAAAGACGGAGAGCGCCAACGGCGTCCATCTCGAAGGAGTCGC11.2658227848101267No Hit
GAAGATAAACTATGCTGCTGGGTAAATGCCAATTGAAAGGACAGGATATAG11.2658227848101267No Hit
AAGAAACAACATTTCAAGTTAAATAAAAATCTGGATAATCATTATATCGAC11.2658227848101267No Hit
CATTGGGGATTGAGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGGTTT11.2658227848101267No Hit
GGATGTGGTCATTCCATCCTGGTGGGGCTGCGTTAAACTTATTTCCCCGCT11.2658227848101267No Hit
CATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTG11.2658227848101267No Hit
AACATAGACTATCACTATTAAAATGCACATAGAAAGACAAGAAAGGCACAA11.2658227848101267No Hit
TTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTA11.2658227848101267No Hit
GGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAA11.2658227848101267No Hit
TACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA11.2658227848101267No Hit
GATATAGCTATTTTTTCTTTACATTTAGCTAGAGTTTCTAGTATTTTAAGA11.2658227848101267No Hit
ATATAGAGACGATATTAAGCAGTATCGTTTCAAATACTTGCATGTTTGGTA11.2658227848101267No Hit
AGTATCCAACCTGCAGAGTTTTATCGCTTCCATGACGCAGAAGTTAACACT11.2658227848101267No Hit
GCTAATGATTAAAAATTGTAAAGTACTAGAATTTATGTGTGATTTTAAAAA11.2658227848101267No Hit
TCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGAAGAA11.2658227848101267No Hit
GTTATATGTTGATTGTAATAACTTGATGACATCATCTTTTGAAAGTTTCTT11.2658227848101267No Hit
GTCATACATTTAGGCTTCACTTAATTAATACCAATTATACTATACTGACAA11.2658227848101267No Hit
TTAGTTAAGCTACCCTAAAGCATATTGGTTTTAAATTACTTTACACAGTTA11.2658227848101267No Hit
TCCTCACATTACTTACTTATCCTCACGTTACTTATTTATCCTCACATTACT11.2658227848101267No Hit
CTTTAATATGCATTTTGTAATATACTGTGCCTATTCATTACAGCTGGGCTC11.2658227848101267No Hit
CTCTGGTACTTCGTGTGAAAACTCTCGGAGAACTCGGAAGAATTATTCCGA11.2658227848101267No Hit
GGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAA11.2658227848101267No Hit
GAACGTAAATGCCAGATCAACGAAAGGTGAGAACTTTCAAACAATAAACCA11.2658227848101267No Hit
TTAGCTGTACCATACTCAGGCACACAAAAATACTGATAGCAGTCGGCGTGT11.2658227848101267No Hit
TTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGA11.2658227848101267No Hit
CTCGGCGTCTTCCCGGAGTCGGGTTGTTTGGGAATGCAGCCCAAAGCGGGT11.2658227848101267No Hit
ATTCGTATGACATAATACTGTGGGGTAAGATGGAAGTCATGAAAATAGGAC11.2658227848101267No Hit
TAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACC11.2658227848101267No Hit
CTTGGGAAGTAGCGACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGC11.2658227848101267No Hit
ATTTAACCAACGCACATCACACGGCAAATGCTCTTAACAACTGCCTTGAAA11.2658227848101267No Hit
AGATAGAAGAAGAGAGATATAGAACAGCTCGTCCGATATATTAAATTGATT11.2658227848101267No Hit
CACCTGCGATATGCCAATGCAAGTGACCACACTGCTACACTACAGTAGATG11.2658227848101267No Hit
TGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTT11.2658227848101267No Hit
AAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTG11.2658227848101267No Hit
GTCTGATAAAAGTTAAAAATTGTAAATTTTTACATAACAGAATTTCTGTTC11.2658227848101267No Hit
GAAATTTCACGCGGCGGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATA11.2658227848101267No Hit
AAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGG11.2658227848101267No Hit
GAACGTAGTATTAAAATTTCGATCAAATAATAATATAGTAATTGCTGCAGC11.2658227848101267No Hit
TACTGTAGGCATGGGTGATGCTGGTATTAAATCTGCCATTCAAGGCTCTAA11.2658227848101267No Hit
TCCGTAAATTCAGCGCCTTCCATGATGAGACAGGCCGTTTGAATGTTGACG11.2658227848101267No Hit
TGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG11.2658227848101267No Hit
ATGTTTTTTATTTATTGTTTTAACCTGTATCAAAATGCATTTTTCAATACT11.2658227848101267No Hit
CCTAGGGTAGGAATTGACACCTTCATCAATATGCCACATTTATTCCAGAAC11.2658227848101267No Hit
GTGTCATTTATGTCATGCCTATGATATTTATGTCATGTTTATGTCATTCAT11.2658227848101267No Hit
TTTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCT11.2658227848101267No Hit
GTTCCAAGTATCGGCAACAGCTTTATCAATACCATGAAAAATATCAACCAC11.2658227848101267No Hit
GTTCTTACTTAACTGTTTTAGTTTTTTAAGTGTTCCTAGCACAATTCCTTT11.2658227848101267No Hit
GGTCAGTGTAGTGAAATTTGCAGATTATTACATAGCTTTATACCTATTCAT11.2658227848101267No Hit
CCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGAT11.2658227848101267No Hit
GCAGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGT11.2658227848101267No Hit
CGTTGGGAGGATTAATGGAATTCACAGCTTGCGTCCTGTCTCTTATACACA11.2658227848101267No Hit
GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTCCTG11.2658227848101267No Hit
TGGCCGTCAACATACATATCACCATTATCGAACTCAACGCCCTGCATACGA11.2658227848101267No Hit
GGCTAAACCAGCCCGAACGTAGCTGCAATTCACACAGAAGCAGAGTAATAG11.2658227848101267No Hit
ACTCCGTGCTGATCCGACTTCATATACAGGCTTCAGCGGGGTGCTATATCT11.2658227848101267No Hit
GTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGA11.2658227848101267No Hit
AGTAACATCATGCATGAAACTGAAGTTATTGTCACTGATAGTTTATCGGAA11.2658227848101267No Hit
TTGCATTCAGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGT11.2658227848101267No Hit
AGCTGAACCTGAGAATCTACTTTTTAATAATAAAAAAAAAGAAGGAGGTAA11.2658227848101267No Hit
AGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGA11.2658227848101267No Hit
ATATATACGTCATCAATTTAGCTGTCGCAGATTTCATCTTTCTTGCAATGT11.2658227848101267No Hit
GGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGG11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG50.045.030
GTATGTT50.045.042
GGGTCAG50.045.015
CTGAGGG50.045.011
GCCTTCG50.045.036
CGTTATG50.045.028
TGGTATC50.045.022
CGCCTTC50.045.035
TGCGCCT50.045.033
TTCGTAT50.045.039
TATGTTT50.045.043
TATGCGC50.045.031
TATCGTT50.045.025
AGTGGTA50.045.020
TGTTTCT50.045.045
GTATCGT50.045.024
AGGGTCA50.045.014
TAAGATT50.045.03
TGCTGAG50.045.09
ATGCGCC50.045.032
Produced by FastQC (version 0.11.2)