FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n01 lacz_15hpf_30apr.341000000334d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n01 lacz_15hpf_30apr.341000000334d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72205180
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACT140300.012.17179834
TCCGAGC146400.011.95677319
CTCCGAG147450.011.64243418
CCGAGCC156200.011.14898620
GACCGTA155150.010.86162932
CGAGCCC159500.010.81957221
ACCGTAC162750.010.79680533
CGTACTA163250.010.75008935
CGAGACC166700.010.58143329
GCCCACG162550.010.50581924
GAGACCG167300.010.32833330
AGCCCAC171350.010.13697423
GTACTAG172350.010.11721636
CCACGAG176250.010.09744326
GAGCCCA172150.010.03759422
CCCACGA182950.09.59262225
ACGAGAC197350.09.21166828
CTAGATC149550.09.17733239
CACGAGA196350.09.00649227
TAGACCG132850.08.8745445
Produced by FastQC (version 0.11.2)