FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n01 gfp_15hpf_30apr.34100000033579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n01 gfp_15hpf_30apr.34100000033579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17890048
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGAGC34550.011.33100336
TCCGAGC35800.010.80988619
CTCCGAG35950.010.63931218
GAGACTC35250.010.59525130
AGACTCC36400.010.50775331
CGAGCCC38400.010.31230821
CGAGACT38500.010.10991929
CCGAGCC39100.010.07017320
TCCTGAG42050.010.0590735
TAGACCG34800.09.8921685
GCATCTC34750.09.58260941
GCCCACG42000.09.42839624
CTCGTAT40500.09.22238545
CTGAGCA43650.09.17506137
GACTCCT42050.09.09589132
AGCCCAC43000.09.05215723
GAGCCCA43650.08.96890522
AGACCGT43450.08.9585086
CCCACGA46300.08.89292325
CCACGAG45100.08.82996626
Produced by FastQC (version 0.11.2)