FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n01 gdna_4jun.34100000033550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n01 gdna_4jun.34100000033550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83358331
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC241600.028.0879745
CTCCGAG399700.019.31401445
TCTCCGA598550.012.93868444
CCTTTGG265050.011.5499661
ATCTCCG734300.010.61718643
CTTTGGG274850.010.2982092
TAGACCG168300.010.2004015
AGACCGT191350.09.124526
CGGGACT198050.09.08823910
TCGGGAC198050.09.0087119
ATACACA2654150.08.82408145
CATCTCC905150.08.66528642
TATACCG172700.08.54655
TATACAC3302150.08.50361845
TTTGGGA328950.08.3788233
GGGAAAC327250.08.2985936
TACACAT1965700.08.270020545
GGCTATT270150.07.8547971
GTGTAGG210300.07.79796271
TATACTG322250.07.7919855
Produced by FastQC (version 0.11.2)