##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H5TT7BCXX l01n01 dnfgfr_18hpf_4jun.34100000033543.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9779519
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.55120164907906	38.0	32.0	38.0	32.0	38.0
2	35.76289130375431	38.0	32.0	38.0	32.0	38.0
3	35.72653184681169	38.0	32.0	38.0	32.0	38.0
4	35.813570278865456	38.0	32.0	38.0	32.0	38.0
5	35.87879996960996	38.0	32.0	38.0	32.0	38.0
6	37.70996763746765	38.0	38.0	40.0	32.0	40.0
7	37.55778776031827	38.0	38.0	40.0	32.0	40.0
8	37.34579492099765	38.0	38.0	40.0	32.0	40.0
9	37.46126624428052	38.0	38.0	40.0	32.0	40.0
10	37.41239247042723	38.0	38.0	40.0	32.0	40.0
11	37.38399659533357	38.0	38.0	40.0	32.0	40.0
12	37.3361710325426	38.0	38.0	40.0	32.0	40.0
13	37.33337181511688	38.0	38.0	40.0	32.0	40.0
14	37.292562650576166	38.0	38.0	40.0	32.0	40.0
15	37.28410027118921	38.0	38.0	40.0	32.0	40.0
16	37.25257060188748	38.0	38.0	40.0	32.0	40.0
17	37.23993388631895	38.0	38.0	40.0	32.0	40.0
18	37.23295614027643	38.0	38.0	40.0	32.0	40.0
19	37.19128404985971	38.0	38.0	40.0	32.0	40.0
20	37.1563602463475	38.0	38.0	40.0	32.0	40.0
21	37.17197931718319	38.0	38.0	40.0	32.0	40.0
22	37.15318524356873	38.0	38.0	40.0	32.0	40.0
23	37.15622731547431	38.0	38.0	40.0	32.0	40.0
24	37.11741978312021	38.0	38.0	40.0	32.0	40.0
25	37.07508170902884	38.0	38.0	40.0	32.0	40.0
26	37.003482073095824	38.0	38.0	40.0	32.0	40.0
27	36.90806347428744	38.0	38.0	40.0	32.0	40.0
28	36.880141753392984	38.0	38.0	40.0	32.0	40.0
29	36.91176979154087	38.0	38.0	40.0	32.0	40.0
30	36.89659941352944	38.0	38.0	40.0	32.0	40.0
31	36.87328088426435	38.0	38.0	40.0	32.0	40.0
32	36.814271438094245	38.0	38.0	40.0	32.0	40.0
33	36.813000618946596	38.0	38.0	40.0	32.0	40.0
34	36.789705710475125	38.0	38.0	40.0	32.0	40.0
35	36.768918900817106	38.0	38.0	40.0	32.0	40.0
36	36.76046102062893	38.0	38.0	40.0	32.0	40.0
37	36.68624152169447	38.0	38.0	40.0	32.0	40.0
38	36.67461957996094	38.0	38.0	40.0	32.0	40.0
39	36.62809510365489	38.0	38.0	40.0	32.0	40.0
40	36.62630789919218	38.0	38.0	40.0	32.0	40.0
41	36.60800955548018	38.0	38.0	40.0	32.0	40.0
42	36.58032813270264	38.0	38.0	40.0	32.0	40.0
43	36.54606612042985	38.0	38.0	40.0	32.0	40.0
44	36.50822029181599	38.0	38.0	40.0	32.0	40.0
45	36.46209256303914	38.0	38.0	40.0	32.0	40.0
46	36.453764955106685	38.0	38.0	40.0	32.0	40.0
47	36.40703883289147	38.0	38.0	40.0	32.0	40.0
48	36.40576187847275	38.0	38.0	40.0	32.0	40.0
49	36.38027412186632	38.0	38.0	40.0	32.0	40.0
50	36.35122760127569	38.0	38.0	40.0	32.0	40.0
51	35.91171048392053	38.0	38.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.4219178683812075
2201	2	0.3051652575182686
2201	3	0.2874431019125865
2201	4	0.39673709305386495
2201	5	0.3280874255790067
2201	6	0.2574723031486954
2201	7	0.30189802946616595
2201	8	0.34318274321483955
2201	9	0.2589784949638272
2201	10	0.17263018224517168
2201	11	0.12519191214897063
2201	12	0.28794968582881353
2201	13	0.29649302520348897
2201	14	0.29893880892981173
2201	15	0.3038743971531517
2201	16	0.29541150173180597
2201	17	0.2535722910729987
2201	18	0.21621295787096528
2201	19	0.155095064122591
2201	20	0.2679739324154369
2201	21	0.19912459260923754
2201	22	0.20826339678865935
2201	23	0.20924529312805618
2201	24	0.2969689890968752
2201	25	0.20361218616585575
2201	26	0.09010979494495075
2201	27	0.0296760365316473
2201	28	-0.010395694794716803
2201	29	0.11454137869746717
2201	30	0.120316710910636
2201	31	0.06575097076331105
2201	32	-0.179861130192144
2201	33	0.028571392133414975
2201	34	0.07491668407816121
2201	35	-0.01505278628864204
2201	36	0.002341750161242828
2201	37	0.10758236130171639
2201	38	0.09027415807468486
2201	39	0.16071139806724233
2201	40	0.0895667458046745
2201	41	0.047587162281061524
2201	42	-0.037197328531242135
2201	43	-0.021597638840475497
2201	44	-0.1541179521290985
2201	45	0.01010997706428185
2201	46	-0.1984663517393912
2201	47	-0.2701753861062457
2201	48	-0.7239403637935027
2201	49	-0.17282447554417502
2201	50	-0.08989327744582454
2201	51	0.12154038219553343
1101	1	0.2284152466608944
1101	2	0.26978913397396553
1101	3	0.24590531853337438
1101	4	0.34763573598859665
1101	5	0.24126946381012004
1101	6	0.23946272432159788
1101	7	0.29362330681381366
1101	8	0.24891027069160998
1101	9	0.17790995961603073
1101	10	0.2635028040813481
1101	11	0.09380696656133125
1101	12	0.24447510886237467
1101	13	0.09905143612166967
1101	14	0.15743850671286452
1101	15	0.14822933255126713
1101	16	0.07506879296840197
1101	17	0.15491892322333456
1101	18	0.13130996630821556
1101	19	0.1723694486834546
1101	20	0.24778725093165832
1101	21	0.014039903857430147
1101	22	0.1287815273945938
1101	23	0.2056142279462776
1101	24	0.169477399464526
1101	25	0.10052096270572974
1101	26	-0.025365909424913013
1101	27	0.191591563027103
1101	28	0.2165456002022026
1101	29	0.12812878128476513
1101	30	0.17809044213740322
1101	31	0.20300060728617808
1101	32	0.2729457902052985
1101	33	0.08079692002714722
1101	34	-0.14921468418414463
1101	35	0.1155156392046095
1101	36	0.10293622050148343
1101	37	-0.06210882868029444
1101	38	-0.025671666542187666
1101	39	0.15529469274937924
1101	40	-0.2690517841565452
1101	41	-0.19030881334353467
1101	42	-0.3456265797924516
1101	43	-0.23701416460570357
1101	44	-0.10951613366033541
1101	45	-0.2466223560901213
1101	46	0.0935996636105969
1101	47	0.19940685968627037
1101	48	0.07871270806418096
1101	49	0.050416119154292005
1101	50	0.006010272421271168
1101	51	0.0013290311155884638
2202	1	0.20999480295319017
2202	2	0.23244935498896524
2202	3	0.26968858015283814
2202	4	0.23590611282318008
2202	5	0.1837749452669044
2202	6	0.13362110446956166
2202	7	0.13175362484279418
2202	8	0.2536742126861
2202	9	0.06192560555206228
2202	10	0.03717130891104148
2202	11	0.06048192482322179
2202	12	0.07989800951818182
2202	13	-0.011275984610648493
2202	14	0.07304489853650153
2202	15	0.005518805584607378
2202	16	0.004720572994386885
2202	17	0.06251614067588918
2202	18	0.0373971659133332
2202	19	0.06376580805267196
2202	20	0.1449830815326294
2202	21	0.09226620364926674
2202	22	0.013139104637033938
2202	23	-0.10724576420356868
2202	24	-0.020863168897349738
2202	25	-0.24855977308524047
2202	26	0.018167547589918343
2202	27	-0.0103662812350791
2202	28	0.019538305652794463
2202	29	-0.12412810224735438
2202	30	-0.18378078018123745
2202	31	-0.1639566118731537
2202	32	-0.014494430236908329
2202	33	-0.13516948501796833
2202	34	-0.1779489325576975
2202	35	-0.19072139332274674
2202	36	-0.044715450870675966
2202	37	-0.10212168161913837
2202	38	-0.026327562971033558
2202	39	-0.016563657454113923
2202	40	0.05947410968011013
2202	41	-0.12246504965226279
2202	42	-0.03196351978388634
2202	43	-0.08771737164780546
2202	44	-0.07170462210115858
2202	45	0.0012500443313285814
2202	46	0.03684777189737787
2202	47	0.028471036784893045
2202	48	-0.16469463310058075
2202	49	-0.1933868068265454
2202	50	-0.1792238250526239
2202	51	-0.17888087518551998
1102	1	0.1606086598247316
1102	2	0.2354424680397713
1102	3	0.2638167565589953
1102	4	0.16070340968506258
1102	5	0.1682479446618288
1102	6	0.10901072607067164
1102	7	0.07474426247611632
1102	8	0.12045655438215164
1102	9	0.028999615423956016
1102	10	0.010045355269788558
1102	11	-0.06799889991300034
1102	12	-0.1401975495961878
1102	13	-0.03321932954852258
1102	14	-0.11229503472499403
1102	15	-0.14562436842946624
1102	16	-0.09708026433668948
1102	17	-0.09786862452943268
1102	18	-0.07850456045704135
1102	19	-0.03733845035424821
1102	20	-0.07473723184544667
1102	21	-0.2696459563613658
1102	22	-0.13603018836462866
1102	23	-0.141706579833631
1102	24	-0.07700926830380439
1102	25	-0.03354772153522845
1102	26	-0.0544521796208528
1102	27	-0.10555195865618572
1102	28	-0.10970206338439681
1102	29	-0.12513817479664624
1102	30	-0.1562652438255938
1102	31	-0.06603646919666772
1102	32	-0.13343015626640664
1102	33	-0.17924052461109596
1102	34	-0.1549706764559815
1102	35	-0.1568338434949652
1102	36	-0.10987202547062935
1102	37	-0.1337917684654002
1102	38	-0.3070939779998838
1102	39	-0.15808960345135148
1102	40	-0.09865822116098855
1102	41	-0.16202899397448078
1102	42	-0.22567319508866035
1102	43	-0.1732028834559216
1102	44	-0.21352070747062157
1102	45	-0.19559230512873427
1102	46	-0.11943791083454158
1102	47	-0.14978604709775567
1102	48	-0.15412743584869304
1102	49	-0.2566948267569842
1102	50	-0.22690183679019071
1102	51	-0.18353623774196848
2203	1	0.08424766263409111
2203	2	0.1483489330212464
2203	3	0.015983922998074718
2203	4	0.035081563522069814
2203	5	0.07331008354855584
2203	6	-0.05704814502390576
2203	7	-0.12719340959132808
2203	8	0.030903322095660712
2203	9	-0.24476085103130174
2203	10	-0.13916640533238223
2203	11	-0.13163008217110672
2203	12	-0.22281440227138916
2203	13	-0.22695820438316616
2203	14	-0.25623934314067043
2203	15	-0.23135095914343395
2203	16	-0.20157007322338671
2203	17	-0.2670223596369681
2203	18	-0.2417919198726466
2203	19	-0.24651911938583737
2203	20	-0.16713619615573094
2203	21	-0.23526711260921473
2203	22	-0.26635966596496274
2203	23	-0.3607154940365547
2203	24	-0.2977017615504636
2203	25	-0.24920947611198585
2203	26	-0.3445571851594593
2203	27	-0.24248260334784533
2203	28	-0.2560476123764275
2203	29	-0.275688865517445
2203	30	-0.34209660632403427
2203	31	-0.3109231154578822
2203	32	-0.30501518864817
2203	33	-0.35328549482332505
2203	34	-0.3492173923804742
2203	35	-0.4120744684219204
2203	36	-0.3145755991175889
2203	37	-0.43708407983772446
2203	38	-0.29839327203967514
2203	39	-0.3007372935178694
2203	40	-0.3615955439588987
2203	41	-0.4483411637825725
2203	42	-0.4312344921200548
2203	43	-0.4476175074782702
2203	44	-0.49916481701371396
2203	45	-0.4045616513365502
2203	46	-0.3277545655449501
2203	47	-0.3360492510863793
2203	48	-0.4234198257442543
2203	49	-0.4372523780484343
2203	50	-0.48674685098451675
2203	51	-0.47500026263882233
1103	1	0.08859809243005401
1103	2	0.09546813196036652
1103	3	0.13376359434159468
1103	4	0.09206128607586095
1103	5	0.05368156647936928
1103	6	-0.020176549863315074
1103	7	-0.01951867605580304
1103	8	-0.015518468612604863
1103	9	-0.13799153490338512
1103	10	-0.18933676784504172
1103	11	-0.11934276045530368
1103	12	-0.06860012882454214
1103	13	-0.09395749467794445
1103	14	-0.08315407353788373
1103	15	-0.18124339234183395
1103	16	-0.13279876954991465
1103	17	-0.07347393615438591
1103	18	-0.15308953392363378
1103	19	-0.05390857448036712
1103	20	-0.025532514159415598
1103	21	-0.20251270475824157
1103	22	-0.15367668802355183
1103	23	-0.2136041444498531
1103	24	-0.20189193544344874
1103	25	-0.1943764172503748
1103	26	-0.24702113460318031
1103	27	-0.18702847105166853
1103	28	-0.22878295850794927
1103	29	-0.19476581377232804
1103	30	-0.1701182621550501
1103	31	-0.25490129388364124
1103	32	-0.19056368107450794
1103	33	-0.2260903455751091
1103	34	-0.2284162464374333
1103	35	-0.29968742167777407
1103	36	-0.3223290704943693
1103	37	-0.2823355903253528
1103	38	-0.2612247113470687
1103	39	-0.17194279524427714
1103	40	-0.23372664599558135
1103	41	-0.32033545275674413
1103	42	-0.3029369649442657
1103	43	-0.3774952110364822
1103	44	-0.33078557881926685
1103	45	-0.3553238530603764
1103	46	-0.3156030671403087
1103	47	-0.22419024560762324
1103	48	-0.2649950697765888
1103	49	-0.31338755486470404
1103	50	-0.3344278443852886
1103	51	-0.35451428116941486
2204	1	-0.05868983146164197
2204	2	0.09383431039788803
2204	3	0.024031946319666986
2204	4	0.02315621581333005
2204	5	0.060213412979145176
2204	6	-0.22066264630367982
2204	7	-0.2378846192588
2204	8	-0.07157369505557654
2204	9	-0.24566832485140822
2204	10	-0.28953391503236503
2204	11	-0.17172879348904502
2204	12	-0.15884847733264706
2204	13	-0.16790973249273833
2204	14	-0.31397059775265745
2204	15	-0.21858123554322617
2204	16	-0.209607425374692
2204	17	-0.19099287545699184
2204	18	-0.1640627849329661
2204	19	-0.24124317040400456
2204	20	-0.1474980133290842
2204	21	-0.17151796563840804
2204	22	-0.13125802695405753
2204	23	-0.16299402965378817
2204	24	-0.2192602623420683
2204	25	-0.24019354325071873
2204	26	-0.26808476030995365
2204	27	-0.24458329625163344
2204	28	-0.19021046266289687
2204	29	-0.2437708412957491
2204	30	-0.2886586194672418
2204	31	-0.11150522534050111
2204	32	-0.2098978038319146
2204	33	-0.30612249110696155
2204	34	-0.2762513961651081
2204	35	-0.25571078391436686
2204	36	-0.2751419978571832
2204	37	-0.2486362644489546
2204	38	-0.31400708809542266
2204	39	-0.26551111717019893
2204	40	-0.30876852068568894
2204	41	-0.3764292504920803
2204	42	-0.34362460837939324
2204	43	-0.3686906285928302
2204	44	-0.2397709342154073
2204	45	-0.2793426193128212
2204	46	-0.3585724854656718
2204	47	-0.31820704096006125
2204	48	-0.4242109887216685
2204	49	-0.44719191432207595
2204	50	-0.38959372044573115
2204	51	-0.40538617972788415
2205	1	-0.02522354312196029
2205	2	-0.017983438353979864
2205	3	0.041326727581477485
2205	4	-0.029543886475799752
2205	5	0.013550837123688098
2205	6	-0.23936286034736298
2205	7	-0.28990293824681146
2205	8	-0.2438453673731047
2205	9	-0.3322409515348781
2205	10	-0.2747792938423075
2205	11	-0.3295168029080813
2205	12	-0.2264224065209106
2205	13	-0.22587375168420465
2205	14	-0.20641734636949138
2205	15	-0.37236492548313294
2205	16	-0.39679991103344747
2205	17	-0.28472742535954865
2205	18	-0.3480572103511008
2205	19	-0.26302938163827605
2205	20	-0.3356069231372345
2205	21	-0.4477193160054398
2205	22	-0.29058242790166133
2205	23	-0.41249333546831224
2205	24	-0.39784882175699465
2205	25	-0.44742296995361386
2205	26	-0.4465486381200421
2205	27	-0.2650848700510693
2205	28	-0.2950477897174082
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1210	11	0.4079481124337434
1210	12	0.2919887344904595
1210	13	0.35425740052624377
1210	14	0.4326673597615809
1210	15	0.3012823079760949
1210	16	0.4384291297754075
1210	17	0.5134699801048797
1210	18	0.384816350735818
1210	19	0.4817970714493711
1210	20	0.47960625023764436
1210	21	0.539882790004981
1210	22	0.5935250366639835
1210	23	0.561308786299378
1210	24	0.5634649550175581
1210	25	0.45500722842869834
1210	26	0.5451728393872557
1210	27	0.5548624749527633
1210	28	0.647569859334638
1210	29	0.5711434223645853
1210	30	0.5908275835571999
1210	31	0.600438688417924
1210	32	0.6261226298271936
1210	33	0.601408602785142
1210	34	0.5962000165596066
1210	35	0.6299464716457663
1210	36	0.5960883610015841
1210	37	0.6381589962186496
1210	38	0.6395041204732763
1210	39	0.6836708442680361
1210	40	0.6612623934000794
1210	41	0.6115686120781234
1210	42	0.6026666062666095
1210	43	0.7448927590223207
1210	44	0.6945618810187
1210	45	0.6182743082941755
1210	46	0.7816266695528
1210	47	0.7933027061262479
1210	48	0.7501517451240716
1210	49	0.7309282141442495
1210	50	0.7788327101976407
1210	51	0.9061279432329101
1211	1	0.09330128052454967
1211	2	0.11566704706399378
1211	3	0.12533702501897181
1211	4	0.13291588523699005
1211	5	0.060107237610068864
1211	6	0.24609941230472998
1211	7	0.264911296422099
1211	8	0.31872643435214343
1211	9	0.32323845973325405
1211	10	0.4437412932051643
1211	11	0.3221216743153761
1211	12	0.3478226250903447
1211	13	0.3378924885845791
1211	14	0.42219346463198804
1211	15	0.36823041502086085
1211	16	0.2673509746114391
1211	17	0.43939719304103164
1211	18	0.39495356132787407
1211	19	0.3439236229765825
1211	20	0.43724971857519535
1211	21	0.37428399655097166
1211	22	0.4135405302078752
1211	23	0.45784880008277895
1211	24	0.48917236080882986
1211	25	0.4808263239850845
1211	26	0.5110527568811349
1211	27	0.6256763311171554
1211	28	0.5833867082504796
1211	29	0.5247372739762639
1211	30	0.42619258324277354
1211	31	0.4509614661130499
1211	32	0.5321864376142003
1211	33	0.5789303825130077
1211	34	0.5244115909858778
1211	35	0.5720557114830598
1211	36	0.5687640146778037
1211	37	0.6105877862447002
1211	38	0.5704426103882838
1211	39	0.5900479387467001
1211	40	0.6526824376644313
1211	41	0.5727018343799273
1211	42	0.6017854524973387
1211	43	0.623376713452231
1211	44	0.6648870696968885
1211	45	0.6591332819600026
1211	46	0.7168205957563885
1211	47	0.7943554654009048
1211	48	0.7764637052401469
1211	49	0.6466169163573667
1211	50	0.6460657996501666
1211	51	0.786189299110994
1208	1	0.04736436696223478
1208	2	0.009710561342025414
1208	3	0.011475444081824548
1208	4	0.08237948722658217
1208	5	-0.036979168263869155
1208	6	0.13394112235067723
1208	7	0.18311246710506168
1208	8	0.37229338215405505
1208	9	0.3162028470931162
1208	10	0.2662975153438296
1208	11	0.3603790779089522
1208	12	0.31008296819091896
1208	13	0.22902510093222617
1208	14	0.31605795462953523
1208	15	0.19976298309578766
1208	16	0.321213624347898
1208	17	0.35070032315454114
1208	18	0.3581148711983744
1208	19	0.268435493603171
1208	20	0.304057954815228
1208	21	0.31255360096310625
1208	22	0.3110329023993117
1208	23	0.29232820563329653
1208	24	0.3565911488528286
1208	25	0.3580476666035821
1208	26	0.37798613388522284
1208	27	0.38702990801950676
1208	28	0.37035318856872834
1208	29	0.4374997895892534
1208	30	0.440960092383726
1208	31	0.29708464175642746
1208	32	0.4398412425886633
1208	33	0.35355086846359995
1208	34	0.34207130796997376
1208	35	0.43235347165229143
1208	36	0.342345823178114
1208	37	0.5464393926087183
1208	38	0.43273277753601036
1208	39	0.43373038435721867
1208	40	0.3253631637547514
1208	41	0.4598443141197919
1208	42	0.5145153314751028
1208	43	0.5646701272659769
1208	44	0.5780132891450975
1208	45	0.5861344593659226
1208	46	0.6946862325231606
1208	47	0.7419120598055216
1208	48	0.6403911059658469
1208	49	0.5003017916764634
1208	50	0.5331911630767863
1208	51	0.5813593091694642
1209	1	-0.028419671098021126
1209	2	-0.008389951901165205
1209	3	-0.11052892065712427
1209	4	0.01905443119536443
1209	5	-0.019696952839126425
1209	6	0.02686984693720973
1209	7	0.17003519128518008
1209	8	0.048376151811638124
1209	9	0.08291202577149903
1209	10	0.17987998865250887
1209	11	0.18631128843919953
1209	12	0.2186601525963141
1209	13	0.15349622934552798
1209	14	0.23161556637811742
1209	15	0.24046594662015508
1209	16	0.3019887483798058
1209	17	0.3222737586039699
1209	18	0.3048788230480568
1209	19	0.15363356254233196
1209	20	0.3851155338142149
1209	21	0.103921154552026
1209	22	0.26600786782341856
1209	23	0.28400172258566414
1209	24	0.2572056359708128
1209	25	0.3145933794036324
1209	26	0.26115111743884256
1209	27	0.3195149613745869
1209	28	0.32798114357785835
1209	29	0.4369425485321443
1209	30	0.3404445644804497
1209	31	0.30337049905686087
1209	32	0.30229211486155094
1209	33	0.27577140319439053
1209	34	0.289982854474232
1209	35	0.37103731314471844
1209	36	0.3249429064353251
1209	37	0.4987129490922584
1209	38	0.4237248298401184
1209	39	0.42675571243999855
1209	40	0.39843712602001347
1209	41	0.4921413689247274
1209	42	0.5453906175700496
1209	43	0.4780513589916353
1209	44	0.528211655593374
1209	45	0.4802938336480693
1209	46	0.5815360412869381
1209	47	0.6976776962100786
1209	48	0.5755159165826882
1209	49	0.5765400205065134
1209	50	0.5537336587404695
1209	51	0.6306598319418271
1214	1	-0.08252082832854057
1214	2	-0.04282659076568507
1214	3	-0.0888085014473532
1214	4	0.005210446604429819
1214	5	-0.05239656098643053
1214	6	0.10216594647805266
1214	7	0.06543280541615815
1214	8	-0.797171617702034
1214	9	-0.058641580589331
1214	10	0.16488219847136065
1214	11	0.12816512164065585
1214	12	0.2095257497711316
1214	13	0.1882624527309389
1214	14	0.28215239510043233
1214	15	0.2507204732319579
1214	16	0.2456491219816641
1214	17	0.2662229932361342
1214	18	0.3815349952565512
1214	19	0.3338028371842299
1214	20	0.4311471855178297
1214	21	0.37159095921874297
1214	22	0.2602842711423179
1214	23	0.2263319406985218
1214	24	0.2587275515430676
1214	25	0.29969633090554737
1214	26	0.3033904770082003
1214	27	0.2989819256517734
1214	28	0.31334292703176203
1214	29	0.3190470721846026
1214	30	0.34948173847322295
1214	31	0.33327008547964
1214	32	0.30951868010128436
1214	33	0.26229162808238016
1214	34	0.268190287945977
1214	35	0.30680894316297724
1214	36	0.33246176163126506
1214	37	0.42694952243952144
1214	38	0.42544955958540953
1214	39	0.4259194102016792
1214	40	0.4006163523000694
1214	41	0.33459513627322224
1214	42	0.40806412551670945
1214	43	0.3488495110323697
1214	44	0.2868923551725828
1214	45	0.22174344923870137
1214	46	0.41464186125721625
1214	47	0.5309753162277815
1214	48	0.47241200729943955
1214	49	0.5763580116810658
1214	50	0.5878254707491664
1214	51	0.5555258710404516
1215	1	-0.23099113572855146
1215	2	-0.10384159140059523
1215	3	-0.10932933486387952
1215	4	-0.06914300390896955
1215	5	-0.16793786723881254
1215	6	-0.009987778284298088
1215	7	-0.017003002839580006
1215	8	-0.6757222191889198
1215	9	-0.10258861715932
1215	10	-0.01184741075183382
1215	11	0.06143757287841112
1215	12	0.10035969338957784
1215	13	0.11683944888234521
1215	14	0.18288689352593934
1215	15	0.18114541535137363
1215	16	0.23936826997307037
1215	17	0.2576275567578321
1215	18	0.13426510601830444
1215	19	0.19748397845598475
1215	20	0.30793812939262466
1215	21	0.19531229545163598
1215	22	0.17235162425660633
1215	23	0.18951473792600382
1215	24	0.258983911219687
1215	25	0.3589838985793108
1215	26	0.23855264247280417
1215	27	0.17210540875719005
1215	28	0.3012657907210894
1215	29	0.27424194946338787
1215	30	0.22145819171712589
1215	31	-0.056106601123453004
1215	32	0.236490034103646
1215	33	0.12418404167279107
1215	34	0.17269934319268998
1215	35	0.28876828978921054
1215	36	0.2939796584916152
1215	37	0.40947916951769514
1215	38	0.3038513366930502
1215	39	0.28800177752756184
1215	40	0.33259087286354827
1215	41	0.335444078130692
1215	42	0.3264430378968015
1215	43	0.3530699020886914
1215	44	0.46765946957687987
1215	45	0.3862081247121125
1215	46	0.36738742547611736
1215	47	0.561466105248563
1215	48	0.387699429622856
1215	49	0.4418398600582947
1215	50	0.524622548089134
1215	51	0.4662218196790704
1212	1	-0.31060630149469404
1212	2	-0.1627326986350539
1212	3	-0.26457818080285733
1212	4	-0.05839709553273309
1212	5	-0.20180370715230822
1212	6	-0.11114203861730942
1212	7	-0.12902583475515428
1212	8	-0.8032391748229628
1212	9	-0.151566914091255
1212	10	-0.19294721947998994
1212	11	-0.19035299262245076
1212	12	0.030300785073372083
1212	13	-0.005148577632454021
1212	14	0.012027598918898263
1212	15	-0.0747172847731008
1212	16	0.02289188388601815
1212	17	-0.17574607964994726
1212	18	-0.04613587602082703
1212	19	0.09290519388487439
1212	20	0.14014059946310198
1212	21	0.025850797730335273
1212	22	-0.013726980810282896
1212	23	0.026799915868771507
1212	24	-0.0190750132012667
1212	25	0.020824702936778294
1212	26	0.19513818290804608
1212	27	0.10213670300290545
1212	28	0.21489038382692627
1212	29	0.17888011831789896
1212	30	0.111590100008371
1212	31	-0.0669700582510444
1212	32	0.036043190990014295
1212	33	0.07407151170460224
1212	34	0.09971772915999111
1212	35	0.171196836308944
1212	36	-0.010930796291454214
1212	37	0.25000504630788356
1212	38	0.20735980894914263
1212	39	0.16870873392777241
1212	40	0.06978707204617507
1212	41	0.11152640722238516
1212	42	0.12296420396702246
1212	43	0.09033404020815539
1212	44	0.11535367954347464
1212	45	0.17594739195063624
1212	46	0.2625701350944212
1212	47	0.33035371169241046
1212	48	0.3617249346085103
1212	49	0.32677631126117035
1212	50	0.37772083148988855
1212	51	0.1392975986807201
1213	1	-0.3686631504409661
1213	2	-0.229191650501825
1213	3	-0.3023072568918437
1213	4	-0.13714647409169345
1213	5	-0.3974884796148288
1213	6	-0.16677771371477945
1213	7	-0.05930372983142718
1213	8	-0.47453582837648867
1213	9	-0.1721934862269734
1213	10	-0.1344215315886288
1213	11	-0.08130347643082558
1213	12	-0.05875301893560447
1213	13	-0.038890147160522304
1213	14	-0.06364599056185227
1213	15	-0.07737654462612653
1213	16	0.036338721439896915
1213	17	0.06751648596006987
1213	18	-0.01939022936164747
1213	19	-0.007936960530969372
1213	20	-0.08719077573249479
1213	21	-0.08859460218629067
1213	22	-0.013507121217237739
1213	23	-0.002675843511532605
1213	24	0.03403227858083824
1213	25	0.026166605051550107
1213	26	-0.08836560031316054
1213	27	-0.18473432348359609
1213	28	-0.019394531617095367
1213	29	-0.04929063002177969
1213	30	-0.013620802241760543
1213	31	-0.1458754358053227
1213	32	-0.01702261626140711
1213	33	-0.10389418610134982
1213	34	-0.01646023427254306
1213	35	-0.05005975943374352
1213	36	-0.09078017845761366
1213	37	0.0070195293646264645
1213	38	-0.051945053609983916
1213	39	0.12577432150001755
1213	40	0.13487757565975755
1213	41	0.1327218871542044
1213	42	0.13344640307999356
1213	43	-0.042638891570589976
1213	44	-0.1671941392364218
1213	45	0.041216914524866866
1213	46	0.045191458913791394
1213	47	0.13225788492834312
1213	48	0.08700154655907255
1213	49	-0.01360093527681272
1213	50	0.11789202576321145
1213	51	-0.2529776056303916
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	41.0
15	78.0
16	137.0
17	100.0
18	136.0
19	255.0
20	636.0
21	1660.0
22	4007.0
23	8545.0
24	16366.0
25	29608.0
26	48915.0
27	74201.0
28	107670.0
29	146688.0
30	193212.0
31	247493.0
32	310690.0
33	387184.0
34	487804.0
35	634136.0
36	877739.0
37	1358572.0
38	2503997.0
39	2339646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.672792308354612	32.23978026561374	18.95727110515139	20.130156320880264
2	14.355389053388004	23.539255867287544	42.398363355089344	19.706991724235106
3	15.344180015397486	29.458187053984965	34.6016506537796	20.59598227683795
4	12.618033668118034	22.063753851288595	42.31586441010033	23.002348070493035
5	12.093232806235154	38.735187282728326	37.34048678672233	11.831093124314192
6	22.924742503762076	42.530175356359415	21.48164486727506	13.063437272603448
7	20.239351264551075	34.91604596463752	27.890801980220292	16.95380079059111
8	19.485815202158715	38.747488501223835	24.98172967402589	16.78496662259156
9	19.894270873649308	19.611925699004214	31.82537914185759	28.668424285488886
10	15.985808298527596	28.938614357067493	34.87775164379267	20.19782570061224
11	28.34854147734669	30.316613731207028	25.95308624074456	15.381758550701727
12	16.818107311821777	29.900171981873548	33.34540277492175	19.936317931382924
13	23.36390982010465	26.432649703937383	29.412622440837836	20.790818035120132
14	18.10065954292142	26.672866710977043	30.388240707602638	24.8382330384989
15	20.27679315057357	34.269433882389755	26.426265951088528	19.027507015948146
16	18.77314277977992	34.395309048267045	28.588309350531745	18.243238821421283
17	17.829663618475497	32.96552846828675	31.207527695033498	17.997280218204253
18	17.771954026655575	32.71724822544482	31.666263271521334	17.844534476378275
19	18.33270412674988	32.53161302936179	31.676048866241995	17.459633977646327
20	18.27493952155924	31.953854985491105	31.87882061263142	17.892384880318232
21	18.387208528520222	31.765764277086923	31.96132610243697	17.88570109195588
22	18.91653278958326	31.656272978568524	31.26355323328111	18.163640998567104
23	18.34517628116475	32.123737373995596	31.318227409752975	18.212858935086686
24	18.5301956057348	32.463161020495996	30.88074168064912	18.12590169312008
25	18.694480447270134	31.892507918491308	31.166742080869593	18.24626955336897
26	18.15745743732386	32.2758205183711	31.39763826830338	18.169083776001663
27	18.285735729947454	32.1382063882692	31.399785613177905	18.17627226860544
28	18.06883344671655	31.993393540111736	31.607975811489297	18.329797201682414
29	17.970300993331062	31.857210973259527	31.841780766518273	18.330707266891142
30	18.10428508804983	31.732491137856577	31.966756238215805	18.196467535877787
31	18.292985575261934	31.40055252206167	31.730026803976756	18.57643509869964
32	18.304589867496265	31.857054421402815	31.385179484672936	18.453176226427985
33	18.206938398504057	31.896466482656255	31.369733010386298	18.52686210845339
34	18.248361703678885	31.889012128306106	31.382668206892383	18.47995796112263
35	18.2152619162558	31.669747765713225	31.585469592113885	18.52952072591709
36	18.03106631793597	31.70744701193296	31.80051957847187	18.460967091659196
37	18.368633467556023	31.749935758599168	31.517020417875358	18.364410355969447
38	18.11412004426807	31.74044831903115	31.88086436650347	18.264567270197308
39	17.932431140430932	31.833819809898355	32.08071369665438	18.153035353016335
40	17.935844007507583	31.58146500133699	32.246840183670955	18.235850807484475
41	18.065086974291834	31.68491555651266	31.953487596859514	18.296509872335996
42	17.93300137307858	31.769569846425433	31.99277180677426	18.304656973721723
43	18.288254617498563	31.331000191730045	31.87474659679172	18.505998593979676
44	18.068150665285955	31.37459572232836	32.075256769813606	18.481996842572084
45	18.01070859739093	31.48703319374786	32.16155089579845	18.34070731306276
46	18.008114601620058	31.35332936308619	32.18045509503234	18.458100940261417
47	17.92142385229089	31.375353053363884	32.16642982355947	18.536793270785758
48	17.75160653436456	31.32559996302445	32.615981126109936	18.306812376501057
49	17.684404254746948	31.79042975414675	32.10043184121054	18.424734149895762
50	17.526535168313924	31.47125464987935	32.42976020948768	18.572449972319042
51	17.44236057511194	31.616247636320356	32.4792693586539	18.462122429913805
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	112.5
2	200.0
3	583.0
4	966.0
5	2513.5
6	4061.0
7	7520.0
8	10979.0
9	18687.5
10	26396.0
11	40293.0
12	54190.0
13	74645.0
14	95100.0
15	117999.0
16	140898.0
17	176540.5
18	212183.0
19	241629.0
20	271075.0
21	294708.5
22	318342.0
23	346457.0
24	374572.0
25	407475.0
26	466530.5
27	492683.0
28	525928.5
29	559174.0
30	568018.5
31	576863.0
32	578593.5
33	580324.0
34	582073.0
35	583822.0
36	588489.0
37	593156.0
38	597723.0
39	602290.0
40	594372.0
41	586454.0
42	571911.5
43	557369.0
44	536161.5
45	514954.0
46	482238.5
47	449523.0
48	416286.5
49	383050.0
50	348396.0
51	313742.0
52	281816.5
53	249891.0
54	218004.0
55	186117.0
56	165569.0
57	145021.0
58	128156.5
59	111292.0
60	98690.5
61	86089.0
62	76248.0
63	66407.0
64	58409.0
65	50411.0
66	45676.0
67	40941.0
68	36485.5
69	32030.0
70	27808.5
71	23587.0
72	19523.5
73	15460.0
74	13060.5
75	9936.5
76	9212.0
77	7502.5
78	5793.0
79	3982.0
80	2171.0
81	1654.5
82	1138.0
83	742.5
84	347.0
85	220.5
86	94.0
87	71.5
88	49.0
89	37.0
90	25.0
91	15.5
92	6.0
93	4.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11990364761293475
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0035482317688630693
7	1.022545178346706E-5
8	0.0
9	0.0
10	1.022545178346706E-5
11	0.0
12	0.0
13	0.0
14	1.9428358388587415E-4
15	1.4315632496853884E-4
16	1.1247996961813766E-4
17	5.930762034410895E-4
18	2.658617463701436E-4
19	7.157816248426942E-5
20	1.022545178346706E-5
21	3.067635535040118E-5
22	2.658617463701436E-4
23	0.0
24	0.0
25	0.0019837376459926094
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	2.1473448745280827E-4
33	0.0
34	0.0
35	0.0
36	5.419489445237542E-4
37	0.0
38	1.022545178346706E-4
39	1.4315632496853884E-4
40	7.566834319765624E-4
41	2.045090356693412E-4
42	3.578908124213471E-4
43	0.0014724650568192566
44	4.090180713386824E-4
45	8.896143051616342E-4
46	0.0011350251479648437
47	0.0019428358388587413
48	8.48712498027766E-4
49	0.0023518539101974236
50	0.002382530265547825
51	0.0028119992404534414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	9779519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.88448328969315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	49.70570556593476	18.333692663491284
2	21.055960177107615	15.532764226019408
3	12.784591003381058	14.146590996893101
4	7.562961584849506	11.158237207878916
5	4.050735283359224	7.470463893501689
6	1.9952629206881216	4.415654511399922
7	0.9004948622765713	2.3250001390066313
8	0.4854036367964977	1.4323089864141363
9	0.23242703311662513	0.7715655917156805
>10	0.9378915807885545	6.8314197548209705
>50	0.14016810095652746	3.6867695345349434
>100	0.13553301438078255	10.028040921529897
>500	0.010744059581911889	2.7396717880249972
>1k	0.0021211767823510926	1.1278197847684512
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.07529000148166796
2	1.022545178346706E-5	0.0	0.076056910365428
3	1.022545178346706E-5	0.0	0.076363673918932
4	1.022545178346706E-5	0.0	0.10606861134990381
5	1.022545178346706E-5	0.0	0.10776603634595934
6	1.022545178346706E-5	0.0	0.10800122173697908
7	1.022545178346706E-5	0.0	0.10882948333143992
8	1.022545178346706E-5	0.0	0.10924872685456206
9	1.022545178346706E-5	0.0	0.11320597669476382
10	1.022545178346706E-5	0.0	0.1170916483724813
11	1.022545178346706E-5	0.0	0.1184311825561155
12	1.022545178346706E-5	0.0	0.12070123285204518
13	1.022545178346706E-5	0.0	0.12161129806077374
14	1.022545178346706E-5	0.0	0.12866685979136602
15	2.045090356693412E-5	0.0	0.12903497605557082
16	2.045090356693412E-5	0.0	0.12912700512162203
17	2.045090356693412E-5	0.0	0.12918835783232283
18	4.090180713386824E-5	0.0	0.1292292596394567
19	4.090180713386824E-5	0.0	0.12925993599480712
20	4.090180713386824E-5	0.0	0.12934173960907483
21	4.090180713386824E-5	0.0	0.12939286686799217
22	4.090180713386824E-5	0.0	0.1294746704822599
23	4.090180713386824E-5	0.0	0.129607601355445
24	4.090180713386824E-5	0.0	0.13013932484818527
25	4.090180713386824E-5	0.0	0.13097781189442959
26	7.157816248426942E-5	0.0	0.13147885903181947
27	7.157816248426942E-5	0.0	0.1350986689631668
28	7.157816248426942E-5	0.0	0.145129837162748
29	1.329308731850718E-4	0.0	0.15903645158826318
30	1.329308731850718E-4	0.0	0.1732498295672824
31	1.6360722853547296E-4	0.0	0.18828124368897897
32	1.6360722853547296E-4	0.0	0.20826177647387362
33	1.9428358388587415E-4	0.0	0.23556373273573067
34	2.045090356693412E-4	0.0	0.27253896638474756
35	2.1473448745280827E-4	0.0	0.3259976283087133
36	2.1473448745280827E-4	0.0	0.40599133761077616
37	2.1473448745280827E-4	0.0	0.5070597030385646
38	2.1473448745280827E-4	0.0	0.6223925737042896
39	2.4541084280320943E-4	0.0	0.7435744027901576
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCGAG	2125	0.0	13.234806	18
GAGACCC	2110	0.0	12.902367	30
AGACCCG	2105	0.0	12.82613	31
TCCGAGC	2145	0.0	12.796861	19
TATCTCG	1885	0.0	12.413525	42
CCGAGCC	2255	0.0	12.172625	20
ACCCGTT	2255	0.0	12.072786	33
GCCCACG	2335	0.0	11.8518715	24
GACCCGT	2265	0.0	11.6220875	32
GTATCTC	2150	0.0	11.511379	41
CGAGACC	2405	0.0	11.506852	29
CCACGAG	2565	0.0	11.490804	26
CGAGCCC	2365	0.0	11.416185	21
CCCACGA	2565	0.0	11.315429	25
TAGACCG	1735	0.0	11.1527815	5
ATCTCCG	2630	0.0	11.035714	16
AGCCCAC	2495	0.0	10.911476	23
ACGAGAC	2565	0.0	10.789075	28
GAGCCCA	2590	0.0	10.685043	22
CCCGTTT	2560	0.0	10.546648	34
>>END_MODULE