FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n01 dnfgfr_18hpf_4jun.34100000033543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n01 dnfgfr_18hpf_4jun.34100000033543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9779519
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG21250.013.23480618
GAGACCC21100.012.90236730
AGACCCG21050.012.8261331
TCCGAGC21450.012.79686119
TATCTCG18850.012.41352542
CCGAGCC22550.012.17262520
ACCCGTT22550.012.07278633
GCCCACG23350.011.851871524
GACCCGT22650.011.622087532
GTATCTC21500.011.51137941
CGAGACC24050.011.50685229
CCACGAG25650.011.49080426
CGAGCCC23650.011.41618521
CCCACGA25650.011.31542925
TAGACCG17350.011.15278155
ATCTCCG26300.011.03571416
AGCCCAC24950.010.91147623
ACGAGAC25650.010.78907528
GAGCCCA25900.010.68504322
CCCGTTT25600.010.54664834
Produced by FastQC (version 0.11.2)