FastQCFastQC Report
Wed 19 Aug 2015
H5TT7BCXX l01n01 dnfgfr_15hpf_30apr.3410000003351c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TT7BCXX l01n01 dnfgfr_15hpf_30apr.3410000003351c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24923413
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25800.012.20943145
TATGCCG26000.011.94236243
AACCGCG36100.011.7793117
ACCGCGG32700.011.7656138
GCGGTAG31800.011.1081411
GGCGAAT48250.011.09816338
ACTAAGG53400.010.99682133
TCCGAGC55100.010.73933319
GCGAATC43600.010.4756539
CGAGACT57800.010.47105929
ACTACCC36300.010.4128318
CTCCGAG56150.010.37808118
CCGCGGT34200.010.3285599
CGTCCAG30000.010.27461310
CCGAGCC58000.010.12478220
ATGCCGT31350.09.97610244
GCCCACG61250.09.95484724
GAGACTA58350.09.90969330
CGAGCCC59900.09.87875321
AGGCGAA60650.09.83072737
Produced by FastQC (version 0.11.2)