FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_wt-hu-3.352000000b0e3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_wt-hu-3.352000000b0e3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17309
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2611.507886070830204Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC253.7333106E-545.020
ACGTCTG206.8421755E-445.022
CACGTCT206.8421755E-445.021
GAGCACA406.171831E-945.016
AGAGCAC406.171831E-945.015
TCTGAAC206.8421755E-445.025
AGCACAC406.171831E-945.017
GCACACG351.1219163E-745.018
CGTCTGA206.8421755E-445.023
CACACGT302.0437565E-644.99999619
GTCGCCG650.041.5384645
AAGAGCA509.549694E-1040.514
TCTCGGT700.038.57142637
GATCTCG700.038.57142635
CGGTGGT700.038.57142640
CTCGGTG700.038.57142638
GTGGTCG700.038.57142642
AGATCTC700.038.57142634
TCGGTGG751.8189894E-1236.039
GTCTGAA250.002050381936.024
Produced by FastQC (version 0.11.2)