FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_wt-3.352000000b0de8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_wt-3.352000000b0de8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34150
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG15134.430453879941435Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT420.12298682284040995Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG950.045.00000418
ACACGTC551.8189894E-1245.020
ACACGTA206.9374684E-445.020
ACGTCTG406.486516E-945.022
CACGTCA206.9374684E-445.021
CACGTAG206.9374684E-445.021
ACGTAGA206.9374684E-445.022
AGAGCAC1050.045.015
GGTAGAT206.9374684E-445.031
AGCACAC1000.045.017
GTGGTCG2550.043.23529442
GAGCACA1050.042.85714316
CGGTGGT2650.042.4528340
TGGTCGC2600.042.40384343
CACACGT800.042.187519
GTCGCCG2650.041.60377545
GGTGGTC2800.040.1785741
TCGGTGG2750.040.09090839
CTCGGTG2750.040.09090838
GGTCGCC2750.040.09090844