Basic Statistics
Measure | Value |
---|---|
Filename | H5TH7BCX2_l02n02_wt-3.352000000b0de8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 34150 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1513 | 4.430453879941435 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 42 | 0.12298682284040995 | Illumina Single End PCR Primer 1 (100% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 95 | 0.0 | 45.000004 | 18 |
ACACGTC | 55 | 1.8189894E-12 | 45.0 | 20 |
ACACGTA | 20 | 6.9374684E-4 | 45.0 | 20 |
ACGTCTG | 40 | 6.486516E-9 | 45.0 | 22 |
CACGTCA | 20 | 6.9374684E-4 | 45.0 | 21 |
CACGTAG | 20 | 6.9374684E-4 | 45.0 | 21 |
ACGTAGA | 20 | 6.9374684E-4 | 45.0 | 22 |
AGAGCAC | 105 | 0.0 | 45.0 | 15 |
GGTAGAT | 20 | 6.9374684E-4 | 45.0 | 31 |
AGCACAC | 100 | 0.0 | 45.0 | 17 |
GTGGTCG | 255 | 0.0 | 43.235294 | 42 |
GAGCACA | 105 | 0.0 | 42.857143 | 16 |
CGGTGGT | 265 | 0.0 | 42.45283 | 40 |
TGGTCGC | 260 | 0.0 | 42.403843 | 43 |
CACACGT | 80 | 0.0 | 42.1875 | 19 |
GTCGCCG | 265 | 0.0 | 41.603775 | 45 |
GGTGGTC | 280 | 0.0 | 40.17857 | 41 |
TCGGTGG | 275 | 0.0 | 40.090908 | 39 |
CTCGGTG | 275 | 0.0 | 40.090908 | 38 |
GGTCGCC | 275 | 0.0 | 40.090908 | 44 |