H5TH7BCX2_l02n02_wt-2.352000000b0db1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5TH7BCX2_l02n02_wt-2.352000000b0db1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26147
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	3177	12.150533522010173	Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAG	196	0.7496079856197652	Illumina Single End PCR Primer 1 (100% over 28bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA	194	0.7419589245420125	Illumina Single End PCR Primer 1 (100% over 27bp)
GTGACTGGAGTTCAGACGTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGA	111	0.4245228898152752	Illumina Single End PCR Primer 1 (100% over 29bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGAA	110	0.4206983592763988	Illumina Single End PCR Primer 1 (100% over 26bp)
AGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	101	0.3862775844265116	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCGGAAGAGCGTCGTGTAGGGCAAGAGTGTAGATCTCGGTGGTCGCCG	95	0.36333040119325355	Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	88	0.33655868742111905	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGG	82	0.31361150418786093	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	54	0.20652464909932305	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	52	0.19887558802157032	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTGTTAGATCGGAAGAGCGTCGTGTA	51	0.195051057482694	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	42	0.16063028263280682	Illumina Single End PCR Primer 1 (98% over 51bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	39	0.14915669101617776	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCGGAAGAGCGTCGTTTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	38	0.1453321604773014	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGGA	38	0.1453321604773014	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGAA	25	3.7256905E-5	45.123077	31
AGGTCGC	45	3.45608E-10	45.123077	43
TTTAGAT	30	2.04567E-6	45.123074	31
AACCGTG	20	6.8794354E-4	45.03647	26
GGGTAGA	30	2.0730677E-6	45.03647	29
TTCCGAG	20	6.8794354E-4	45.03647	26
TCTGGGT	50	2.0008883E-11	45.03647	37
CTCGGAG	25	3.7684098E-5	45.03647	38
GGGTGGT	50	2.0008883E-11	45.03647	40
ATGAGTG	50	2.0008883E-11	45.03647	26
AATGAGT	20	6.8794354E-4	45.03647	25
CATGAGT	35	1.14467184E-7	45.036465	25
AGCGAGT	35	1.14467184E-7	45.036465	27
GGCATGA	20	6.944983E-4	44.950195	23
GGGCAAC	20	6.944983E-4	44.950195	22
AACTGGA	20	6.944983E-4	44.950195	3
CACGTCA	20	6.944983E-4	44.950195	21
GCGGTTA	40	6.4483174E-9	44.950195	15
GCGGTGA	20	6.944983E-4	44.950195	15
GAACGGG	20	6.944983E-4	44.950195	2

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content