H5TH7BCX2_l02n02_wt-1.352000000b0d98.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H5TH7BCX2_l02n02_wt-1.352000000b0d98.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181895
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	17370	9.54946535088925	Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA	1306	0.7179966464168889	Illumina Single End PCR Primer 1 (100% over 27bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAG	1279	0.7031529178921906	Illumina Single End PCR Primer 1 (100% over 28bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGAA	1049	0.5767063415706865	Illumina Single End PCR Primer 1 (100% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGCAAGAGTGTAGATCTCGGTGGTCGCCG	852	0.4684021001127024	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	835	0.45905604881937384	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTGTTAGATCGGAAGAGCGTCGTGTA	818	0.44970999752604524	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCGTTAGATCGGAAGAGCGTCGTGT	785	0.4315676626625251	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGACTGGAGTTCAGACGTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGA	631	0.34690343329943096	Illumina Single End PCR Primer 1 (100% over 29bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGGA	480	0.2638885071057478	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	468	0.2572912944281041	Illumina Single End PCR Primer 1 (98% over 51bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	452	0.24849501085791253	Illumina Single End PCR Primer 1 (98% over 51bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	451	0.24794524313477556	Illumina Single End PCR Primer 1 (98% over 51bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	359	0.19736661260617389	Illumina Single End PCR Primer 1 (98% over 51bp)
AGATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	355	0.195167541713626	Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCGTCTAGATCGGAAGAGCGTCGTG	282	0.15503449792462684	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGG	234	0.12864564721405206	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AGATCGGAAGAGCGTCGTTTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	205	0.1127023832430798	Illumina Single End PCR Primer 1 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCCG	60	0.0	45.108997	45
ATGGGCG	20	6.9344114E-4	45.108997	42
GCGCCGG	40	6.6174835E-9	45.108997	45
TTCGTCT	55	1.8189894E-12	45.04685	26
ATCCGAG	30	2.1399883E-6	45.04685	26
AACCGAG	50	2.1827873E-11	45.046844	26
CTTCCTG	25	3.8540355E-5	45.046844	25
CTCCGAG	25	3.8540355E-5	45.046844	26
TCCGATA	45	3.765308E-10	45.046844	27
TCCGAGG	25	3.8540355E-5	45.046844	27
ATCAGTG	50	2.1827873E-11	45.046844	26
ACCGAGT	65	0.0	45.046844	27
TTCCGTC	50	2.1827873E-11	45.046844	26
TTCCGTA	20	6.9816964E-4	45.046844	26
CTGCGTA	45	3.765308E-10	45.046844	25
AATGCGT	100	0.0	45.046844	25
TTGCGGA	25	3.8540355E-5	45.046844	25
TCCGTCT	50	2.1827873E-11	45.046844	27
GCCTAGG	20	6.9816964E-4	45.046844	27
CGAGCAA	25	3.8540355E-5	45.046844	29

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content