FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_gal-ssb_glu_doxrap-1.352000000b0ecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_gal-ssb_glu_doxrap-1.352000000b0ecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699047
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1118941.9633808950864942Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG61250.042.28266518
GTCGCCG160650.041.6146545
GGTCGCC161950.041.14137644
CACACGT50150.040.84686319
GTGGTCG165200.039.6359142
AGCACAC67250.039.57996417
AGAGCAC75400.039.23570615
GAGCACA75700.039.02253316
TGGTCGC169400.038.83954643
GGTGGTC173300.037.89710641
ACACGTC32200.037.22543320
CACGTAG22550.037.09922421
CGGTGGT177550.036.7743940
ACACGTA24700.034.78042220
ACGTCAG22650.034.74955422
CACACGA16150.032.71949419
ACACGAG16100.032.54606220
CTCGGTG201500.032.0986538
TCTCGGT202850.031.7288237
TCGGTGG207500.031.21691139
Produced by FastQC (version 0.11.2)