FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_gal-ssb_gal_doxrap-3.352000000b0ea4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_gal-ssb_gal_doxrap-3.352000000b0ea4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11793622
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9398607.9692226866352Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT240620.20402553176623772Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG220580.18703329647160133Illumina Single End PCR Primer 1 (100% over 41bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT134760.11426515111303381Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1164250.043.08088745
GCACACG256450.042.1563818
GGTCGCC1191450.042.0008644
CACACGT198800.041.36052719
TGGTCGC1200350.041.29493743
GTGGTCG1199400.041.28816642
AGCACAC272550.040.91127417
GGTGGTC1215650.040.7295341
GAGCACA291350.040.5400616
CGGTGGT1223300.040.3699140
AGAGCAC293700.040.2456215
ACACGTC117450.040.13017320
CACGTAG88550.038.85399221
GTGACTG126350.038.305291
ACACGTA90800.038.2131520
CACACGA67950.037.9534719
ACGTCAG86600.037.8317122
ACACGAG67850.037.78224620
TGACTGG128450.037.3985862
CACGTCA90700.036.14789221