Basic Statistics
Measure | Value |
---|---|
Filename | H5TH7BCX2_l02n02_gal-ssb_gal_doxrap-3.352000000b0ea4.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11793622 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 939860 | 7.9692226866352 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 24062 | 0.20402553176623772 | Illumina Single End PCR Primer 1 (100% over 39bp) |
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG | 22058 | 0.18703329647160133 | Illumina Single End PCR Primer 1 (100% over 41bp) |
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 13476 | 0.11426515111303381 | Illumina Single End PCR Primer 1 (100% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 116425 | 0.0 | 43.080887 | 45 |
GCACACG | 25645 | 0.0 | 42.15638 | 18 |
GGTCGCC | 119145 | 0.0 | 42.00086 | 44 |
CACACGT | 19880 | 0.0 | 41.360527 | 19 |
TGGTCGC | 120035 | 0.0 | 41.294937 | 43 |
GTGGTCG | 119940 | 0.0 | 41.288166 | 42 |
AGCACAC | 27255 | 0.0 | 40.911274 | 17 |
GGTGGTC | 121565 | 0.0 | 40.72953 | 41 |
GAGCACA | 29135 | 0.0 | 40.54006 | 16 |
CGGTGGT | 122330 | 0.0 | 40.36991 | 40 |
AGAGCAC | 29370 | 0.0 | 40.24562 | 15 |
ACACGTC | 11745 | 0.0 | 40.130173 | 20 |
CACGTAG | 8855 | 0.0 | 38.853992 | 21 |
GTGACTG | 12635 | 0.0 | 38.30529 | 1 |
ACACGTA | 9080 | 0.0 | 38.21315 | 20 |
CACACGA | 6795 | 0.0 | 37.95347 | 19 |
ACGTCAG | 8660 | 0.0 | 37.83171 | 22 |
ACACGAG | 6785 | 0.0 | 37.782246 | 20 |
TGACTGG | 12845 | 0.0 | 37.398586 | 2 |
CACGTCA | 9070 | 0.0 | 36.147892 | 21 |