FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_gal-ssb_gal_doxrap-2.352000000b0e8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_gal-ssb_gal_doxrap-2.352000000b0e8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55651
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG39567.108587446766455Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT850.1527375968086827Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG720.1293777290614724Illumina Single End PCR Primer 1 (100% over 41bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT650.11679933873605147Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCAG302.1409833E-644.961377
AGAGCAC1050.044.96136515
GAGCACA1100.042.91766716
AGCACAC1050.042.82034717
GCACACG1050.042.82034718
GTCGCCG4950.042.00874345
GGTCGCC5050.041.17688844
GTGGTCG5150.040.37733542
TGGTCGC5250.040.03876543
CAGATAG451.8864739E-839.9656566
ACACGTC403.4026743E-739.34119420
ACGTAGA403.4026743E-739.34119422
CACGTAG403.4026743E-739.34119421
GGTGGTC5350.039.11371241
AAGAGCA1150.039.0968414
CGGTGGT5400.038.7515540
TCGCCGT355.952492E-638.74719245
CACACGT900.037.46780419
TCTCGGT5650.036.6716137
CTCGGTG5650.036.63863438