Basic Statistics
Measure | Value |
---|---|
Filename | H5TH7BCX2_l02n02_gal-ssb_gal_doxrap-2.352000000b0e8a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 55651 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 3956 | 7.108587446766455 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 85 | 0.1527375968086827 | Illumina Single End PCR Primer 1 (100% over 39bp) |
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG | 72 | 0.1293777290614724 | Illumina Single End PCR Primer 1 (100% over 41bp) |
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 65 | 0.11679933873605147 | Illumina Single End PCR Primer 1 (100% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGATCAG | 30 | 2.1409833E-6 | 44.96137 | 7 |
AGAGCAC | 105 | 0.0 | 44.961365 | 15 |
GAGCACA | 110 | 0.0 | 42.917667 | 16 |
AGCACAC | 105 | 0.0 | 42.820347 | 17 |
GCACACG | 105 | 0.0 | 42.820347 | 18 |
GTCGCCG | 495 | 0.0 | 42.008743 | 45 |
GGTCGCC | 505 | 0.0 | 41.176888 | 44 |
GTGGTCG | 515 | 0.0 | 40.377335 | 42 |
TGGTCGC | 525 | 0.0 | 40.038765 | 43 |
CAGATAG | 45 | 1.8864739E-8 | 39.965656 | 6 |
ACACGTC | 40 | 3.4026743E-7 | 39.341194 | 20 |
ACGTAGA | 40 | 3.4026743E-7 | 39.341194 | 22 |
CACGTAG | 40 | 3.4026743E-7 | 39.341194 | 21 |
GGTGGTC | 535 | 0.0 | 39.113712 | 41 |
AAGAGCA | 115 | 0.0 | 39.09684 | 14 |
CGGTGGT | 540 | 0.0 | 38.75155 | 40 |
TCGCCGT | 35 | 5.952492E-6 | 38.747192 | 45 |
CACACGT | 90 | 0.0 | 37.467804 | 19 |
TCTCGGT | 565 | 0.0 | 36.67161 | 37 |
CTCGGTG | 565 | 0.0 | 36.638634 | 38 |