FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_gal-ssb_gal_doxrap-1.352000000b0e60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_gal-ssb_gal_doxrap-1.352000000b0e60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6109
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG821.342281879194631Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC206.495352E-445.015
CGTCTGA206.495352E-445.023
AAGAGCA301.01237376E-437.49999614
AAAGAGT250.001947386836.025
ATCGGAA550.003511860720.4545469
GATCGGA600.00581331518.7499988
AGATCGG600.00581331518.7499987
Produced by FastQC (version 0.11.2)