FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_exo1d-2.352000000b0e2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_exo1d-2.352000000b0e2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11733291
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2401252.046527270141003Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG329100.042.30983745
GGTCGCC333400.041.6897544
GTGGTCG338950.040.3218942
TGGTCGC342750.039.95380443
GCACACG61500.039.4062418
GGTGGTC354550.038.49699441
CGGTGGT359050.037.93298340
CACACGT51650.036.30644619
AGCACAC70650.035.35260417
GAGCACA78600.033.60753316
AGAGCAC80900.032.6516515
CTCGGTG417550.032.38299638
TCTCGGT422550.031.89980337
TCGGTGG426950.031.68409339
ACACGTC33600.031.6565520
ATCTCGG438150.031.00650636
CACGTAG26900.029.42605821
GATCTCG476600.028.48238635
GTGACTG48500.028.0546841
TGACTGG50050.026.736392
Produced by FastQC (version 0.11.2)