FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_wt-hu-3.351000000b0e3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_wt-hu-3.351000000b0e3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17309
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1430.8261598012594604TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGATCGGAAGAGCACA310.1790975792940089TruSeq Adapter, Index 7 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG206.8421755E-445.02
GTGACTG206.8421755E-445.01
TGGAGTT250.002050381936.06
GAGTTCA250.002050381936.08
GACTGGA250.002050381936.03
ACTGGAG250.002050381936.04
GGAGTTC250.002050381936.07
AGTCACT707.003109E-1032.14285729
GTCACTA651.2423698E-831.15384530
CAGTCAC808.367351E-1130.937528
CCAGTCA808.367351E-1130.937527
CTCCAGT909.094947E-1230.00000225
TCCAGTC909.094947E-1230.00000226
TATGCCG300.005009971629.99999845
TCACTAG602.1947199E-729.99999831
CACTAGC553.862335E-628.63636632
AACTCCA952.0008883E-1128.42105323
GAACTCC952.0008883E-1128.42105322
ACTCCAG1003.6379788E-1127.024
TGAACTC1056.91216E-1125.71428521
Produced by FastQC (version 0.11.2)