FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_wt-hu-1.351000000b0e06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_wt-hu-1.351000000b0e06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44041410
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC447517810.16129592581164TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC3680050.8355885971861482TruSeq Adapter, Index 14 (97% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA1116620.2535386582763813TruSeq Adapter, Index 8 (100% over 40bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG1114880.25314357555763994TruSeq Adapter, Index 8 (100% over 38bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG709750.16115514921070875TruSeq Adapter, Index 8 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGC670310.1521999409192394TruSeq Adapter, Index 8 (98% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG659150.14966596210248492TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAACCTCGTATGC606140.137629562722901TruSeq Adapter, Index 8 (98% over 50bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC463360.105210073882739TruSeq Adapter, Index 8 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5388950.041.86040545
TCGTATG5541150.040.6732544
CTCGTAT5540050.040.43926643
TCTCGTA5418850.039.9044342
ATCTCGT5422000.039.857641
AATCTCG5389750.039.7839840
GAATCTC6151800.034.70770639
TGAATCT6207050.034.36182438
TTGAATC6274750.033.996737
CTTGAAT6634550.032.2238436
GGTCGGA90000.030.0521142
AGGTCGG91850.029.5224931
ACTTGAA7788400.028.42630235
CACTTGA8156750.027.18845734
GACGTGT837200.026.9974731
CCTCGTA111750.026.9071342
ACTCGTA135450.026.86476142
ACCTCGT111800.026.83474741
AACCTCG111900.026.34851340
ACACTTG8694600.025.58617833
Produced by FastQC (version 0.11.2)