FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_wt-3.351000000b0deb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_wt-3.351000000b0deb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34150
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC3761.1010248901903368TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTACGATCTCGTATG2750.8052708638360175TruSeq Adapter, Index 16 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCGGAAGAGCACA1470.4304538799414348TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC860.2518301610541728TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCAC740.21669106881405564TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC600.17569546120058566TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAACCGTACGATCTCGTATG550.16105417276720352TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC530.15519765739385066TruSeq Adapter, Index 20 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG302.1064188E-644.9926840
TCACGTG301.11796646E-437.493931
CACGTGG250.0020803335.99414432
ACCCGTA504.652975E-835.99414433
CACCCGT504.652975E-835.99414432
GCATCTC250.0020803335.99414439
AGTCACG702.0008883E-1135.3513929
TGACTGG1300.034.66052
GTGACTG1300.034.66051
CATCTCG754.3655746E-1132.99463340
CCCGTAC551.0757685E-732.7219534
GACTGGA1400.032.1847533
ACTGGAG1400.032.1847534
GAATCTC352.7664847E-432.1376339
TGAATCT352.7664847E-432.1376338
CCAGTCA3100.031.93028827
CAGTCAC3050.031.71614828
CGTATGC1350.031.66151445
TCCAGTC3200.031.63547726
CTGGAGT1500.031.5410565
Produced by FastQC (version 0.11.2)