FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_wt-2.351000000b0db4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_wt-2.351000000b0db4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26147
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC513819.650437908746703TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2530.9676062263357174TruSeq Adapter, Index 8 (100% over 50bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC2140.8184495353195395Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC2040.780204229930776TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC1480.5660305197537002TruSeq Adapter, Index 20 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA1350.5163116227483077TruSeq Adapter, Index 14 (96% over 33bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCA1180.45129460358740964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT1170.44747007304853326TruSeq Adapter, Index 14 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCGCAGATCGGAAGAGCACAC990.37862852334875896TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCCATCTCGTATGC720.2753661987990974TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCCATCTCGTATGC660.2524190155658393TruSeq Adapter, Index 21 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG650.24859448502696294TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCGGAAGAGCACAC650.24859448502696294TruSeq Adapter, Index 14 (97% over 40bp)
AACAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT580.2218227712548285TruSeq Adapter, Index 13 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACAC550.2103491796381994TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCAATCTCGTATGC530.20270011856044673TruSeq Adapter, Index 21 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGCGCACAC500.19122652694381764TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGCAATCTCGTATGC500.19122652694381764TruSeq Adapter, Index 20 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTGAATCTCGTATGC490.1874019964049413TruSeq Adapter, Index 21 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACACG410.15680575209393047Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACAC400.15298122155505411TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACAGATCGGAAGAGCACAC380.1453321604773014TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGCATCTCGTATGC380.1453321604773014TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCGTCACAGATCGGAAGAGCACACG370.14150762993842506Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTGAATCTCGTATGC370.14150762993842506TruSeq Adapter, Index 8 (98% over 50bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC360.1376830993995487TruSeq Adapter, Index 14 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTCCATCTCGTATGC360.1376830993995487TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCAACTCGTATGC360.1376830993995487TruSeq Adapter, Index 20 (97% over 39bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA360.1376830993995487TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTGCATCTCGTATGC350.13385856886067235TruSeq Adapter, Index 21 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTCAATCTCGTATGC340.130034038321796TruSeq Adapter, Index 15 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGC340.130034038321796TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACAGATCGGAAGAGCACAC340.130034038321796TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCACCCACAGATCGGAAGAGCACAC330.12620950778291964Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTGAATCTCGTATGC330.12620950778291964TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG320.12238497724404329Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATNGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC310.11856044670516692TruSeq Adapter, Index 13 (97% over 35bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGT300.11473591616629059Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC270.10326232454966153TruSeq Adapter, Index 13 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTGG406.4173946E-944.97509832
GAAGCGC253.7990852E-544.97509841
TCACGTG502.0008883E-1144.97509831
CGGAAGC253.7990852E-544.97509839
CATAGCG206.926057E-444.97509834
GTGGCCA406.4173946E-944.97509835
TCACATT206.926057E-444.97509831
TCACAGT406.4173946E-944.97509831
CACAGTT406.4173946E-944.97509832
AGTTCGG253.7990852E-544.97509835
GTTCGGA253.7990852E-544.97509836
GGCCATC302.09278E-644.97509437
TGGCCAT302.09278E-644.97509436
ACAGTTC351.1571137E-744.97509433
GTCACAT555.638867E-1140.8864530
ACGTGGC451.816261E-839.97786733
GTCACGT850.039.68390730
CTCGTAT1700.039.68390743
TTCGGAA403.3027572E-739.3532137
ATCTCGT1500.038.97841641
Produced by FastQC (version 0.11.2)