FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_wt-1.351000000b0d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_wt-1.351000000b0d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181895
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC5834032.0734489678111TruSeq Adapter, Index 14 (97% over 38bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC14620.803760411226257TruSeq Adapter, Index 14 (96% over 29bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC14070.7735231864537233Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC13520.7432859616811897TruSeq Adapter, Index 8 (100% over 50bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT11450.629484042991836TruSeq Adapter, Index 14 (96% over 30bp)
AACAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT8900.4892932735919074TruSeq Adapter, Index 13 (96% over 30bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCA8480.46620302922015455Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC8410.4623546551581956TruSeq Adapter, Index 13 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCGGAAGAGCACAC6690.367794606778636TruSeq Adapter, Index 14 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGCGCACAC6660.3661453036092251TruSeq Adapter, Index 14 (97% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGG6540.3595480909315814TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATC6150.3381071497292394TruSeq Adapter, Index 14 (97% over 38bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC5890.3238131889276781TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCGCAGATCGGAAGAGCACAC5510.302922015448473TruSeq Adapter, Index 13 (97% over 35bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC5430.2985238736633772TruSeq Adapter, Index 14 (96% over 31bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATC5160.2836801451386789TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAACTCGTATGC4960.2726847906759394TruSeq Adapter, Index 8 (98% over 50bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA4290.23585035322576214TruSeq Adapter, Index 14 (97% over 38bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG4170.22925314054811843TruSeq Adapter, Index 13 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACAGATCGGAAGAGCACAC3680.20231452211440668TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACAC3630.1995656834987218TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAAATCGTATGC3420.1880205613128453TruSeq Adapter, Index 8 (97% over 40bp)
AGACGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT3040.16712938783364029TruSeq Adapter, Index 13 (96% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA2960.16273124604854447TruSeq Adapter, Index 14 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACACG2810.15448473020148987Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATNGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC2600.1429396080156134TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG2500.13744193078424366TruSeq Adapter, Index 14 (97% over 38bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG2450.13469309216855876TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACAC2420.13304378899914787TruSeq Adapter, Index 13 (97% over 35bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG2420.13304378899914787TruSeq Adapter, Index 14 (97% over 34bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGT2350.12919541493718903Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCGGAAGCGCACAC2080.11435168641249073TruSeq Adapter, Index 14 (97% over 40bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC2030.11160284779680583TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCGTCACAGATCGGAAGAGCACACG1960.10775447373484702Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCATAC1960.10775447373484702TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAACGGAAGAGCACAC1880.10335633194975123TruSeq Adapter, Index 2 (97% over 36bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA1850.1017070287803403TruSeq Adapter, Index 14 (96% over 32bp)
GACGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC1820.10005772561092938TruSeq Adapter, Index 13 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT207.0324185E-444.98075531
AGCGTAC502.1827873E-1144.98075543
TCGCACG207.0324185E-444.98075544
CGAACGC207.0324185E-444.98075539
CGAGTAC302.1617543E-644.98075543
CGCATGC800.044.98075545
AGAAGCG207.0324185E-444.98075540
GCGCATG502.1827873E-1144.98075544
ATCGTAC351.2084638E-744.98075543
AGTATAC453.8198777E-1044.98075545
ACTAGCA253.88763E-544.98075542
GTTCGAA600.044.98075536
AGCATGC207.0324185E-444.98075545
AGCATAC1000.044.98075545
GGGAAGC253.88763E-544.98075539
GTCACCC253.88763E-544.98075530
AACGCGT253.88763E-544.98075541
CACAGTA453.8198777E-1044.98075532
CGTACGC502.1827873E-1144.98075545
TCGTACG351.2084638E-744.98075544
Produced by FastQC (version 0.11.2)