FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_gal-ssb_glu_doxrap-1.351000000b0ec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_gal-ssb_glu_doxrap-1.351000000b0ec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699047
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1006421.7659443763141454TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC209390.3674123059522057TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC139250.038.41924345
TCGTATG143350.037.2873944
CTCGTAT142550.037.0105643
GATCTCG138200.036.17397740
ATCTCGT139800.035.88865741
TCTCGTA144450.034.79563542
CACGATC168150.029.10226837
ACGATCT173000.028.5853838
TCACGAT176700.027.8575936
CGATCTC184000.027.12091339
ATCACGA202400.025.6200135
CATCACG207450.025.02907834
CAGTCAC271300.023.92937528
AGTCACA269700.023.85455129
GTCACAT226350.023.50524330
TCCAGTC285350.023.27003726
CCAGTCA280400.023.19967527
TCACATC227400.023.13960531
ACATCAC226650.023.00802833
CACATCA227800.022.89167432
Produced by FastQC (version 0.11.2)