FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_gal-ssb_gal_doxrap-2.351000000b0e8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_gal-ssb_gal_doxrap-2.351000000b0e8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55651
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAATCTCGTATGCC18993.4123376039963342TruSeq Adapter, Index 12 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAACTTGAATCTCGTATGCC15812.840919300641498TruSeq Adapter, Index 12 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGATCGGAAGAGCAC1010.18148820326678766TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGTATCTCGTATGCC870.1563314226159458TruSeq Adapter, Index 12 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGAAACTCGTATGCC830.14914377100141957TruSeq Adapter, Index 12 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTATCTCGTATGCC640.1150024258324199TruSeq Adapter, Index 12 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4950.039.98003444
GTATGCC4950.039.98003445
TCGTATG5050.039.63367543
CTCGTAT5150.037.5540642
ATCTCGT5000.036.88158440
TCTCGTA5000.036.88158441
AAACTCG250.002095089335.98203339
ACTCGTA250.002095089335.98203341
AATCTCG5000.035.53225739
GAATCTC5050.035.18045438
TGAATCT5200.034.16562737
TTGAATC5300.033.52099636
AACTCGT300.005118246729.98502540
CTTGAAT6150.029.25368535
ACTTGAA2850.028.40686834
GTCACCT4400.027.59985230
AACTTGA2900.027.14161933
CAACTTG3000.026.9865232
CCTTGAA4100.026.87682234
AGTCAAC2950.026.6815929
Produced by FastQC (version 0.11.2)