FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_gal-ssb_gal_doxrap-1.351000000b0e63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_gal-ssb_gal_doxrap-1.351000000b0e63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6109
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC520.8512031429039123TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACGATCAGATCTCGTATGCC130.21280078572597808Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCGATCAGATCTCGTATGCC90.1473236208872156Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACGCCAATATCTCGTATGCC70.11458503846783433Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)