FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n01_exo1d-1.351000000b0df8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n01_exo1d-1.351000000b0df8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7368675
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1598842.1697795058134606TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC187320.25421123879123453TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC198100.040.0828745
TCGTATG204750.038.63720744
CTCGTAT203600.038.5819443
AATCTCG199000.038.01597640
ATCTCGT201250.037.93736341
TCTCGTA208850.036.65374442
CAATCTC232250.032.49596839
CCAATCT233900.032.112938
ACCAATC235050.031.86967537
GACCAAT243900.030.72061736
TGACCAA296700.026.04188235
CTGACCA299050.025.87483434
ACTGACC303450.025.54394333
CACTGAC312400.024.9343332
AGTCACT323050.024.49516329
GTCACTG322750.024.3437530
CAGTCAC370600.024.21019628
TCACTGA323950.024.1008631
CCAGTCA380950.023.65755827
TCCAGTC388250.023.57761626
Produced by FastQC (version 0.11.2)