FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_wt-hu-1.352000000b0e03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_wt-hu-1.352000000b0e03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370130
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG578541.323850777894479Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT67240.15386269973662112Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG83000.040.38936618
CACACGT58750.039.3826419
ACACGTC41950.039.2906520
AGCACAC91000.038.19231417
GAGCACA93450.037.84053416
AGAGCAC94900.037.3566915
CACGTCT25100.037.0888921
GTCGCCG98650.036.7654945
ACACGAG28550.036.0725320
GGTCGCC101750.035.6675344
CACGTAG18400.035.440321
ACGTCTG21600.035.29093622
CACACGA29500.035.2903819
ACGTCAG19550.035.19588522
CGTCTGA21100.035.16808323
GTGGTCG105150.034.27866742
CACGAGA30650.033.82109521
GCGGCGC8900.033.3748551
TGGTCGC108500.033.2410843
AAGAGCA109200.032.83529714
Produced by FastQC (version 0.11.2)