FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_gal-ssb_glu_doxrap-1.352000000b0ecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_gal-ssb_glu_doxrap-1.352000000b0ecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852477
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10284957.4246288227008055Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT501950.36235396745289666Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT218700.15787790154786036Illumina Single End PCR Primer 1 (100% over 47bp)
GAGGAGAACTTCTAGTATATTCTGTATACCTAATATTATAGCCTTTATCAA210580.15201613400982367No Hit
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC209010.15088276270012937Illumina Single End PCR Primer 1 (100% over 50bp)
AGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC201030.14512206011964504Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG195540.14115886999848476Illumina Single End PCR Primer 1 (98% over 51bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT162860.11756742133554887Illumina Single End PCR Primer 1 (97% over 49bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG139150.10045134888150328Illumina Single End PCR Primer 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG802200.042.39454318
AGCACAC868050.042.02494417
GAGCACA1126800.041.97221416
ACACGAG429950.041.85771620
CACACGA430900.041.8037119
AGAGCAC1137450.041.7724615
CACGAGA437550.041.0998421
CACACGT385400.040.9746819
CACGTAG198350.040.6894621
GTCGCCG1415250.040.57629845
ACACGTA203450.040.06738320
AAGAGCA1381850.039.48772414
ACACGTC189300.039.17785620
GCACAAG235750.039.1116918
ACGAGAT461950.038.96303622
TCGCCGT135650.038.70733645
GGTCGCC1500600.038.2503944
CACGTCT95600.037.68298321
AGCACAA247300.037.49846617
GTGGTCG1515550.037.4525942
Produced by FastQC (version 0.11.2)