FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_gal-ssb_gal_dox-1.352000000b0e54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_gal-ssb_gal_dox-1.352000000b0e54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12419293
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6167274.965878492439143Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT722910.5820862749594522Illumina Single End PCR Primer 1 (100% over 51bp)
AGATGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT134820.10855690416515659Illumina Single End PCR Primer 1 (100% over 47bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG550350.040.30156318
CACACGT368000.040.25406319
ACACGTC244400.040.00700420
AGCACAC583800.039.75948317
GAGCACA604900.039.57610316
AGAGCAC607800.039.51191315
ACGTCAG133050.038.94173422
ACACGAG188550.038.6545120
CACACGA190200.038.516319
CACGTCT118600.037.90319421
GTCGCCG959150.037.86146545
CACGAGA193000.037.83316421
CACGTAG131200.037.65684521
CGTCTGA89200.037.51334423
ACACGTA134000.037.4070120
TCGCCGT147750.037.203745
ACGTCTG94600.036.8458222
GGTCGCC990750.036.62244
GTGGTCG998750.035.82349842
AAGAGCA683050.035.56049714
Produced by FastQC (version 0.11.2)