FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_exo_hku-2.352000000b0d8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_exo_hku-2.352000000b0d8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11464
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3603.14026517794836Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGG450.392533147243545Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA280.24424284717376132Illumina Single End PCR Primer 1 (100% over 27bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAG250.21807397069085835Illumina Single End PCR Primer 1 (100% over 28bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATAGATCGGAAGAGCGTCGTG240.20935101186322402Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAAGATCGGAAGAGCGTCGTGT160.13956734124214934Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGAA150.130844382414515Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGTG206.6500355E-445.12719338
GCGGCGG206.796276E-444.93013412
TGGAGAG206.796276E-444.9301346
CTGGAGA206.796276E-444.9301345
GAGCGGC253.6871777E-544.9301310
GCGTGTA253.6871777E-544.9301328
AGCGGCG253.6871777E-544.9301311
CGGGAGA253.6871777E-544.930135
ACGGGAG253.6871777E-544.930134
CGTGTGC351.0906297E-744.9301317
AGCGTGT253.6871777E-544.9301327
GTGTGCT351.0906297E-744.9301318
GTGCTCT302.004068E-644.9301320
AGAGCAC750.041.93478815
AGCACAC554.9112714E-1140.84557717
GCACACG554.9112714E-1140.84557718
CACACGT451.6814738E-839.93789719
AAGAGCA800.039.31386614
GAGCACA800.039.31386616
GACGTGT403.11411E-739.31386615
Produced by FastQC (version 0.11.2)