FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_exo_hku-1.352000000b0d7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_exo_hku-1.352000000b0d7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7166
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1912.665364219927435Illumina Single End PCR Primer 1 (100% over 51bp)
AGGTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAA80.11163829193413341Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT80.11163829193413341Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGACT206.5729546E-445.0000042
AGGTGAC206.5729546E-445.0000041
CGGTGGT405.331458E-945.00000440
CGTGTGC351.0017175E-745.00000419
GGTGGTC405.331458E-945.00000441
ACTAGAT351.0017175E-745.00000430
GTGGTCG405.331458E-945.00000442
TGAACTA206.5729546E-445.00000427
TGTGCTC206.5729546E-445.00000421
ACGTGTG351.0017175E-745.00000418
ACAGATC253.5146342E-545.030
GAACTAG253.5146342E-545.028
GAACAGA452.8376235E-1045.028
GTGTGCT253.5146342E-545.020
AACTAGA301.8777155E-644.99999629
AACAGAT301.8777155E-644.99999629
AGAGCAC650.041.5384615
CACGTCT601.8189894E-1241.24999621
GAGTGTA554.1836756E-1140.90909228
ACGTCTG554.1836756E-1140.90909222
Produced by FastQC (version 0.11.2)