FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_exo1d-3.352000000b0e47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_exo1d-3.352000000b0e47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40020
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG890.22238880559720142Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG351.1515658E-745.10539639
CTCGGTG253.770751E-545.10539638
TCTCGGT253.798865E-545.04887437
ATAAGAT406.544724E-944.992494
TAAGATC600.044.992495
GATCTCG253.8271526E-544.9924935
ATAGATC206.9574645E-444.992495
CACCCGT207.000574E-444.9362519
CGTCTGA2350.042.12062523
TGAACTA750.041.99299227
GAACAGA1300.041.5315328
ACACGTC2550.041.4636720
ACGTCTG2450.041.31963722
GCACACG2750.040.8511418
CACGTCT2550.040.58146321
GTCTGAA2400.040.3057724
GAGCACA2750.040.03411516
CACACGT2700.039.94333619
CTGAACT800.039.41776726
AGCACAC2850.039.41776317
Produced by FastQC (version 0.11.2)