FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n02_exo1d-2.352000000b0e2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n02_exo1d-2.352000000b0e2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18931
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG480.2535523744123395Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGG310.16375257514130265Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGAA310.16375257514130265Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAAA280.1479055517405314Illumina Single End PCR Primer 1 (100% over 27bp)
GTGTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGA270.142623210606941TruSeq Adapter, Index 1 (96% over 26bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAAG230.12149384607257938Illumina Single End PCR Primer 1 (100% over 28bp)
GTGTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAA210.11092916380539856Illumina Single End PCR Primer 1 (100% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC302.0540065E-645.00000420
ACGTCTG253.7466583E-545.00000422
CACGTCT253.7466583E-545.00000421
AGGGCAC302.0540065E-645.00000421
AGAGCAT253.7466583E-545.0000049
AGAGCAG302.0540065E-645.0000049
GTGGGCG302.0540065E-645.00000442
CGGTGGG253.7466583E-545.00000440
TGGGCGC302.0540065E-645.00000443
GTAGGGC253.7466583E-545.00000419
GAGGGCA253.7466583E-545.00000420
GTGTGCA253.7466583E-545.00000420
ATCCCGG206.858461E-445.036
GTAGAGC206.858461E-445.07
GGGCACT206.858461E-445.022
GGGCAAG206.858461E-445.022
AGTAGAT206.858461E-445.031
AGGGATA206.858461E-445.021
GAGCAGC206.858461E-445.010
AGCAGCG206.858461E-445.011
Produced by FastQC (version 0.11.2)