FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_wt-hu-1.351000000b0e06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_wt-hu-1.351000000b0e06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4370130
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC671191.5358582010146151TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC70700.16178008434531696TruSeq Adapter, Index 8 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC47410.10848647523071396TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC104600.032.03963545
CTCGTAT108100.030.91869243
TCGTATG108900.030.77417244
ATCTCGT108750.029.9476241
AATCTCG111150.029.09895740
TCTCGTA112450.029.06257842
GTGACTG19350.024.6392081
GTCACAC136200.024.63824530
GAATCTC131200.024.58347739
TGAATCT133500.024.14309538
CAGTCAC152700.024.00858928
CCAGTCA154400.023.9479127
AGTCACA149800.023.9328629
TTGAATC134750.023.86905737
TCCAGTC156500.023.74099226
CACTTGA140100.023.50287634
CCTACAC29550.023.28987111
CTACACG29600.023.25052812
CTCCAGT163300.022.87634325
ACACTTG145050.022.82486233
Produced by FastQC (version 0.11.2)