FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_exo_hku-2.351000000b0d8e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_exo_hku-2.351000000b0d8e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11464
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC10739.359734822051639TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC1711.491625959525471TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG720.6280530355896721TruSeq Adapter, Index 14 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA700.6106071179344034TruSeq Adapter, Index 14 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC410.3576413119330077TruSeq Adapter, Index 1 (100% over 50bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC290.2529658060013957TruSeq Adapter, Index 14 (96% over 29bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC280.24424284717376132Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT240.20935101186322402TruSeq Adapter, Index 14 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT170.1482903000697837TruSeq Adapter, Index 14 (96% over 30bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA160.13956734124214934TruSeq Adapter, Index 14 (97% over 38bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC150.130844382414515TruSeq Adapter, Index 14 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTGG351.0443546E-745.1754382
GACTGGA351.0443546E-745.1754383
ACTGGAG351.0443546E-745.1754384
ACGATCT554.9112714E-1140.8892438
GTGACTG402.9822695E-739.5285071
TACGATC403.0880983E-739.35589637
CGTACGA403.0880983E-739.35589635
GTACGAT403.0880983E-739.35589636
TCTCGTA657.2759576E-1238.0584542
CGTATGC657.2759576E-1238.0584545
TCGTATG657.2759576E-1238.0584544
CTCGTAT657.2759576E-1238.0584543
GCAGATC301.0739754E-437.48180430
ATCTCGT701.6370905E-1135.3399941
CACCGTA457.773906E-734.98301732
CCGTACG457.773906E-734.98301734
GTCACAG1300.032.8686630
CTGGAGT501.7114307E-631.6228075
ACCGTAC501.7718558E-631.48471533
TGGAGTT501.7718558E-631.4847156
Produced by FastQC (version 0.11.2)