FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_exo_hku-1.351000000b0d71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_exo_hku-1.351000000b0d71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7166
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGATCGGAAGAGCAC620.8651967624895338TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACGGCTACATCTCGTATGCC460.641920178621267Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATCGGAAGAGCACA430.6000558191459671TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCACGGCTACATCTCGTATGCC280.3907340217694669Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGATCGGAAGAGCAC280.3907340217694669TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGATCGGAAGAGCAC260.3628244487859336TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTGCTCTTCCGATCT230.3209600893106335TruSeq Adapter, Index 15 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC200.27909572983533354TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGATCGGAAGAGCAC170.23723137036003347TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC150.20932179737650014TruSeq Adapter, Index 20 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAGGCTACATCTCGTATGCC150.20932179737650014TruSeq Adapter, Index 6 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGATCGGAAGAGCAC120.1674574379012001TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTGACGGCTACATCTCGTATGC80.11163829193413341TruSeq Adapter, Index 11 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCGGAAGAGCACACGT80.11163829193413341TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCC206.6235295E-444.93007321
AGTCACG250.001985544635.94405729
AGTTCAG1500.030.378251
CATCTCG300.00485320929.9533840
ACTCCAG851.3278623E-1029.072424
GAACTCC851.3278623E-1029.072422
AACTCCA902.6011548E-1027.45726623
TGAACTC902.6011548E-1027.45726621
CTCCAGT754.24443E-826.95804225
CTGAACT1008.8402885E-1024.71153820
CGATCTT551.2045058E-424.5073121
CGATCTC551.2045058E-424.5073120
CAGTCAC651.2829363E-524.19311328
CCAGTCA651.2829363E-524.19311327
TCCAGTC651.2829363E-524.19311326
GTCTGAA1102.6720954E-922.46503418
CGTCTGA1102.6720954E-922.46503417
ACGTCTG1154.469257E-921.48829516
TCTGAAC1154.469257E-921.48829519
GTTCAGA2650.020.6342831
Produced by FastQC (version 0.11.2)