FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_exo1d-3.351000000b0e4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_exo1d-3.351000000b0e4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40020
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCAC720.17991004497751123TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG1500.043.6552471
TGACTGG1650.039.587252
GACTGGA1650.039.587253
ACTGGAG1750.037.3251234
CTGGAGT2100.030.0317085
ATCTTAT453.4127508E-529.99416722
GGAGTTC2100.029.9941677
TGGAGTT2150.029.2966276
ACACGTC3400.029.11198645
CCAGTCA554.040574E-628.63079527
CTCCAGT554.040574E-628.63079525
GAGTTCA2200.028.6307958
AACTCCA554.040574E-628.63079523
CAGTCAC506.999346E-526.99475128
GAACTCC607.948385E-626.24489622
ATCTGGG450.001207341824.99513823
GATCTAG551.3380095E-424.5406822
ACTCCAG651.4787845E-524.22605724
TCCAGTC651.4787845E-524.22605726
TGAACTC651.4787845E-524.22605721
Produced by FastQC (version 0.11.2)