FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_exo1d-2.351000000b0e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_exo1d-2.351000000b0e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18931
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC7614.0198616026623TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATCGGAAGAGCAC3671.9386191960276795TruSeq Adapter, Index 25 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCGGAAGAGCAC990.5229517722254503TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAGATCGGAAGAGCAC830.4384343140880038TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG530.27996408008029156TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGATCGGAAGAGCACA380.20072896307643545TruSeq Adapter, Index 25 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA360.19016428080925468TruSeq Adapter, Index 14 (96% over 33bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC320.16903491627489303TruSeq Adapter, Index 14 (96% over 29bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT310.16375257514130265TruSeq Adapter, Index 14 (96% over 30bp)
AGCACACGTCTGAACTCCAGTCACACAGATCGGAAGAGCACACGTCTGAAC280.1479055517405314TruSeq Adapter, Index 25 (96% over 30bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC230.12149384607257938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACACGTCTGAACTCCAGTCACACAGATCGGAAGAGCACACGTCTGAACT220.11621150493898895TruSeq Adapter, Index 25 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG700.041.8842351
TGGAGTT800.039.3634266
GAGTTCA800.039.3634268
TGACTGG750.039.0919532
ACACAGA657.2759576E-1238.0657333
ACTGGAG850.037.14624
GGAGTTC850.037.047937
CTGGAGT900.035.082525
GACTGGA900.035.082523
AGTTCAG950.033.1481489
GTCACAC754.0017767E-1132.990330
CACACAG754.0017767E-1132.990332
TCACACA754.0017767E-1132.990331
TCACAGA1450.032.57662631
CAGTCAC2600.032.0098228
CCAGTCA2600.032.0098227
CTCCAGT2600.032.0098225
AGTCACA2250.031.99059529
TCCAGTC2700.031.65735626
ACTCCAG2650.031.4058624
Produced by FastQC (version 0.11.2)