FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l01n01_dna2-frb-1.351000000b0da8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l01n01_dna2-frb-1.351000000b0da8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13852477
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC10791787.790505625816957TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC701070.506097212794506TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC512940.3702875666207567TruSeq Adapter, Index 1 (100% over 51bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG325970.2353153158095841TruSeq Adapter, Index 1 (100% over 49bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG236800.17094415677427222TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGC198050.14297082030888772TruSeq Adapter, Index 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1307600.040.3146945
TCGTATG1392900.037.8251144
CTCGTAT1402500.037.37856343
TCTCGTA1364550.037.0237442
ATCTCGT1370200.036.8676641
GATCTCG1416700.035.50235440
ACGATCT1425550.035.12732338
CACGATC1426100.035.0790837
TCACGAT1451050.034.56339336
ATCACGA1526700.034.1162635
CATCACG1559350.033.40192834
GTGACTG109200.033.0130651
ACATCAC1605000.032.572333
GTCACAT1623100.032.40584630
CACATCA1615200.032.39027832
TCACATC1620650.032.378531
GACTGGA115250.031.5151463
AGTCACA1793700.031.31874529
TGACTGG116350.031.1008572
CAGTCAC1835200.030.93040528
Produced by FastQC (version 0.11.2)