FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_gal-ssb_gal_dox-3.352000000b0e97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_gal-ssb_gal_dox-3.352000000b0e97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28204596
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG377285413.376734770460812Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG941780.33391011876220456Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT896200.3177496320103291Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT526400.18663624892907527Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC396770.14067565442171198Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC334080.1184487804753523Illumina Single End PCR Primer 1 (100% over 40bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT313690.11121946224650761Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG4542300.043.47744845
GCACACG1087500.042.8175718
CACACGT864350.042.51072719
GGTCGCC4650500.042.3679544
AGCACAC1137050.042.15775317
ACACGTC511400.041.97033320
TGGTCGC4663150.041.94693843
GAGCACA1224650.041.78955516
GTGGTCG4680900.041.7353742
AGAGCAC1233250.041.57899515
GGTGGTC4720300.041.37791441
ACGTCAG373900.040.95634522
CGGTGGT4752200.040.95424340
CACGTAG370900.040.9157921
ACACGTA374350.040.7069220
GTGACTG460350.040.503331
CACACGA241900.040.36114519
TGACTGG459450.039.9018672
ACACGAG244150.039.7094620
CACGTCA388250.039.5391721
Produced by FastQC (version 0.11.2)