FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_gal-ssb_gal_dox-2.352000000b0e7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_gal-ssb_gal_dox-2.352000000b0e7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44041410
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG466523010.592826160651986Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG1102320.25029171409362233Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT1096120.24888394808431427Illumina Single End PCR Primer 1 (100% over 39bp)
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT698550.15861208803260385Illumina Single End PCR Primer 1 (100% over 39bp)
AGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC459000.10422009649554817Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG5751950.043.2804645
GCACACG1471450.042.6854218
GGTCGCC5875850.042.281144
CACACGT1150050.042.27797319
AGCACAC1537700.041.96911617
TGGTCGC5892200.041.80900643
ACACGTC699150.041.75671420
GTGGTCG5906150.041.6732242
GAGCACA1657300.041.52190416
AGAGCAC1669200.041.30051415
GGTGGTC5968150.041.24540741
CGGTGGT6002100.040.86919440
CACGTAG475450.040.36007321
ACGTCAG503900.040.25993722
ACACGTA485100.039.7658120
CACACGA353800.039.68258719
GTGACTG611200.039.5546881
ACACGAG354950.039.31241220
TGACTGG613700.038.865422
CACGTCA527300.038.5792621