Basic Statistics
Measure | Value |
---|---|
Filename | H5TH7BCX2_l02n02_gal-ssb_gal_dox-2.352000000b0e7d.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 44041410 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 4665230 | 10.592826160651986 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG | 110232 | 0.25029171409362233 | Illumina Single End PCR Primer 1 (100% over 41bp) |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 109612 | 0.24888394808431427 | Illumina Single End PCR Primer 1 (100% over 39bp) |
AGATCGGAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 69855 | 0.15861208803260385 | Illumina Single End PCR Primer 1 (100% over 39bp) |
AGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 45900 | 0.10422009649554817 | Illumina Single End PCR Primer 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 575195 | 0.0 | 43.28046 | 45 |
GCACACG | 147145 | 0.0 | 42.68542 | 18 |
GGTCGCC | 587585 | 0.0 | 42.2811 | 44 |
CACACGT | 115005 | 0.0 | 42.277973 | 19 |
AGCACAC | 153770 | 0.0 | 41.969116 | 17 |
TGGTCGC | 589220 | 0.0 | 41.809006 | 43 |
ACACGTC | 69915 | 0.0 | 41.756714 | 20 |
GTGGTCG | 590615 | 0.0 | 41.67322 | 42 |
GAGCACA | 165730 | 0.0 | 41.521904 | 16 |
AGAGCAC | 166920 | 0.0 | 41.300514 | 15 |
GGTGGTC | 596815 | 0.0 | 41.245407 | 41 |
CGGTGGT | 600210 | 0.0 | 40.869194 | 40 |
CACGTAG | 47545 | 0.0 | 40.360073 | 21 |
ACGTCAG | 50390 | 0.0 | 40.259937 | 22 |
ACACGTA | 48510 | 0.0 | 39.76581 | 20 |
CACACGA | 35380 | 0.0 | 39.682587 | 19 |
GTGACTG | 61120 | 0.0 | 39.554688 | 1 |
ACACGAG | 35495 | 0.0 | 39.312412 | 20 |
TGACTGG | 61370 | 0.0 | 38.86542 | 2 |
CACGTCA | 52730 | 0.0 | 38.57926 | 21 |