FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_exo_hku-2.352000000b0d8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_exo_hku-2.352000000b0d8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12555949
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8232616.556740553820345Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT548610.4369323258640187Illumina Single End PCR Primer 1 (100% over 51bp)
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCAT139370.11099917656562637Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG434900.041.33819218
GAGCACA616950.040.6948316
AGCACAC468650.040.66374217
CACACGA212550.040.54686419
AGAGCAC626750.040.31254215
GTCGCCG1346500.040.22726445
ACACGAG211300.040.21772420
CACACGT228000.039.94909319
CACGAGA214750.039.5611521
GGTCGCC1384350.038.95473544
ACACGTC126850.038.82341820
GTGGTCG1365100.038.4964342
TGGTCGC1372600.038.3780843
GGTGGTC1381100.037.99329441
CGGTGGT1378650.037.6655540
CACGTAG106700.037.61957621
GCACAAG144900.037.11133218
ACACGTA110100.036.9684620
CACGTCT73100.036.8535721
ACGAGAT232850.036.50222422
Produced by FastQC (version 0.11.2)