FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_exo_hku-1.352000000b0d7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_exo_hku-1.352000000b0d7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11766765
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6050085.141668079544378Illumina Single End PCR Primer 1 (100% over 51bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT312770.2658079769588328Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG355550.041.1457818
AGCACAC384000.040.61492517
GTCGCCG931750.040.5820745
CACACGA180000.040.57468419
GAGCACA510150.040.42580416
ACACGAG179050.040.40580720
AGAGCAC517900.039.9889715
CACGAGA181250.039.8533221
CACACGT182300.039.58172219
GGTCGCC953200.039.4133644
GTGGTCG949050.038.9133142
TGGTCGC957200.038.6479543
GGTGGTC960100.038.3769541
CACGTAG86650.038.15186721
CGGTGGT966150.037.7521740
ACACGTC97500.037.7120520
ACACGTA90450.037.49382420
GCACAAG116450.036.62460718
ACGAGAT198600.036.51608722
AAGAGCA672250.036.1089814
Produced by FastQC (version 0.11.2)