FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_exo1d-3.352000000b0e47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_exo1d-3.352000000b0e47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9244664
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2652772.8695147817162416Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG328350.042.470645
GGTCGCC334950.041.4923644
GTGGTCG339100.040.67865842
GCACACG84950.040.67607518
TGGTCGC342650.040.29669643
GGTGGTC351150.039.30521441
CGGTGGT357250.038.64038540
CACACGT70350.038.5778119
AGCACAC92250.038.4070917
AGAGCAC103600.037.36717215
GAGCACA104300.037.00921216
ACACGTC43300.036.8221520
CTCGGTG401950.034.2887738
TCTCGGT408050.033.82308637
TCGGTGG411300.033.55707639
CACACGA23000.033.23880819
CACGTAG32800.033.04638721
ATCTCGG423450.032.6532936
ACGTCAG31800.030.9701822
ACACGAG25000.030.76358220
Produced by FastQC (version 0.11.2)