FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_exo1d-1.352000000b0df5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_exo1d-1.352000000b0df5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7368675
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1675612.2739637723199895Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG213850.042.43480745
GGTCGCC217250.041.67727344
GCACACG55650.040.3157518
GTGGTCG222200.040.24136442
TGGTCGC222700.040.1611443
GGTGGTC231650.038.6373741
CACACGT45800.038.2928919
CGGTGGT234100.038.0983540
AGCACAC60650.038.06703617
GAGCACA70450.035.8669716
CTCGGTG247650.035.8644538
AGAGCAC70650.035.8604115
ACACGTC30900.035.5875720
TCTCGGT252300.035.1224237
TCGGTGG255600.034.83201239
ATCTCGG265000.033.77260236
CACGTAG20200.032.39586621
ACGTCAG21750.030.49641822
GATCTCG296500.030.04334335
ACACGTA22150.029.74691220
Produced by FastQC (version 0.11.2)