FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_dna2-frb-3.352000000b0ddb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_dna2-frb-3.352000000b0ddb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13097956
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2328271.7775827006900926Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG311300.041.8111545
GGTCGCC316450.041.09507844
GCACACG118600.040.75630618
GTGGTCG328300.039.27518542
CACACGT96200.039.0336219
TGGTCGC334900.038.46750343
AGCACAC130250.038.1464317
AGAGCAC144450.037.99061215
GGTGGTC342900.037.58263841
GAGCACA146300.037.28099416
CGGTGGT355750.036.13651740
ACACGTC56200.035.73295620
CACGTAG49650.035.14764821
ACACGTA53100.032.77934620
ACACGAG33250.031.855420
CACACGA34200.031.22949419
ACGTCAG47000.030.7139922
CTCGGTG421350.030.3683638
TCTCGGT428350.029.8044537
TCGGTGG437000.029.35594739
Produced by FastQC (version 0.11.2)