FastQCFastQC Report
Tue 16 Jan 2018
H5TH7BCX2_l02n02_dna2-frb-2.352000000b0dce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH5TH7BCX2_l02n02_dna2-frb-2.352000000b0dce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682447
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1535251.5856012431568176Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG82250.041.35652518
GTCGCCG217450.041.2621645
GGTCGCC221700.040.32876644
CACACGT65250.039.21438219
AGCACAC90700.038.51986317
GTGGTCG227150.038.4180842
TGGTCGC232100.037.70560543
GAGCACA101550.037.6365716
AGAGCAC102050.037.539915
GGTGGTC239850.036.31687541
ACACGTC39250.035.06392320
CGGTGGT248300.034.92681540
CACGTAG35550.033.20996521
CACACGA26150.032.58751319
ACACGAG26350.031.74761220
ACACGTA37600.031.63854820
CTCGGTG276200.031.2001938
TCTCGGT281750.030.52998737
TCGGTGG287500.030.07064839
ACGTCAG30650.029.71237622
Produced by FastQC (version 0.11.2)